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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 220 - 227 (8page)
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초록· 키워드
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Amyloid plaque is a product of aggregation of β-amyloid peptide (Aβ) and is an important factor in the pathogenesis of Alzheimer’s Disease (AD). Aβ is a major component of amyloid plaque and vascular deposits in the AD brain. The enzyme β- secretase is required for the production of Aβ; thus, prevention of the formation of Aβ through the inhibition of β-secretase is a major focus in the study of the treatment of AD. In this study, we investigated β-secretase inhibitory activity of an Arctoscopus japonicus peptide. An Alcalase hydrolysate had the highest β-secretase inhibitory activity. A β-secretase inhibitory activity peptide was separated using ion exchange column chromatography (carboxy-methyl: CM, quaternary methyl ammonium: QMA) and reverse phase high performance liquid chromatography (RP-HPLC) on a C18 column. The IC50 value of the purified peptide was 248.2±1.73 μg/mL. The β-secretase inhibitory peptide was identified as a six amino acid residue of Gly-Pro-Val-Gly-Ala- Pro (MW: 497.27 Da). In cell viability experiments, the final purified fraction, the carboxy-methyl ion exchange column fraction (CM-F1) showed no significant cytotoxic effect in SH−SY5Y cells at concentrations below 100 μg/mL in 24 h. The results of this study suggest that peptides separated from Arctoscopus japonicus may be beneficial as β-secretase inhibitor compounds in functional foods.
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참고문헌 (32)
참고문헌 신청
Thies, W. / 2013 / Alzheimer’s Association. Alzheimer’s disease facts and figures / Alzheimers. Dement 9:208~245
Dickson, D. W. / 1997 / The pathogenesis of senile plaques / J Neuropathol. Exp. Neur 56:321~339
Vassar, R. / 1999 / Beta-secretase cleavage of Alzheimer’s amyloid precursor protein by the transmembrane aspartic protease BACE / Science 286:735~741
Wang, R. / 1996 / The profile of soluble amyloid beta protein in cultured cell media Detection and quantification of amyloid beta protein and variants by immunoprecipitation-mass spectrometry / J. Biol. Chem 271:31894~31902
Citron, M. / 2002 / β-secretase as a target for the treatment of Alzheimer’s disease / J. Neurosci. Res 70:373~379
Dickson, D. W. / 1997 / The pathogenesis of senile plaques / J Neuropathol. Exp. Neur 56:321~339
Vassar, R. / 1999 / Beta-secretase cleavage of Alzheimer’s amyloid precursor protein by the transmembrane aspartic protease BACE / Science 286:735~741
Wang, R. / 1996 / The profile of soluble amyloid beta protein in cultured cell media Detection and quantification of amyloid beta protein and variants by immunoprecipitation-mass spectrometry / J. Biol. Chem 271:31894~31902
Citron, M. / 2002 / β-secretase as a target for the treatment of Alzheimer’s disease / J. Neurosci. Res 70:373~379
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