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논문 기본 정보

자료유형
학술저널
저자정보
전희재 (광주과학기술원) 이형빈 (연세대학교) 윤대성 (고려대학교) 김법민 (고려대학교)
저널정보
대한의용생체공학회 Biomedical Engineering Letters (BMEL) Biomedical Engineering Letters (BMEL) Vol.7 No.4
발행연도
2017.1
수록면
317 - 323 (7page)

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Red blood cell (RBC) dysfunction is oftenassociated with a pathological intervention, and it has beenproposed as a critical risk factor for certain lethal diseases. Examining the cell viability of RBCs under various physiologicalconditions is essential and of importance forprecise diagnosis and drug discovery in the field of medicineand pharmacy. In this paper, we report a new analyticalmethod that employs dielectrophoretic (DEP) forcemeasurements in absolute units to assess the viability, andpotentially the functionality of RBCs. We precisely quantifythe frequency-dependent DEP forces of the RBCs byusing a micro-electrode embedded chip combined withoptical tweezers. DEP characteristics are known to be wellcorrelatedwith the viability of biological cells, and DEPforces are measured in both fresh and long-term storedRBCs to investigate the effect that the storage period hason the cell viability. Moreover, we investigate the DEPbehavior of RBCs when exposed to oxidative stress andverify whether EDTA protects the RBCs from an oxidant. From the experiments, it is found that the fresh RBCswithout oxidative stress display very high DEP forces overthe entire frequency range, exhibiting two cutofffrequencies. However, both the RBCs stored for the longtermperiod and exposed to oxidative stress reveals thatthere exist no significant DEP forces over the frequencyrange. The results indicate that the DEP forces can serve asa useful parameter to verify whether the RBCs in certainblood are fresh and not exposed to oxidative stress. Therefore, it is believed that our system can be applied to adiagnostic system to monitor the cell viability of the RBCsor other types of cells.

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