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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국동물유전육종학회 Journal of Animal Breeding and Genomics Journal of Animal Breeding and Genomics Vol.5 No.2
- 발행연도
- 2021.1
- 수록면
- 59 - 70 (12page)
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Sexing of birds at early age is very important for efficient selection and breeding; while characterization and taxonomic identification is relevant in conservation of birds’ genetic resources. This study used the genomic DNA of ten (10) guinea fowl keets to determine their sex using agarose gel electrophoresis and sequencing with chromo helicase DNA (CHD) binding genes, they were also characterize taxonomically using 12S rRNA mitochondria genes. The results of this study shows a double band (ZW) for females and a single band (W) for males under Agarose gel electrophoresis view, the Guinea fowl keets sequenced showed some deletions and were closer to Gallus_CHD12 in the phylogenetic tree. The Taxonomic classification result shows that the sequenced guineafowl keets were most related to the Numida meleagris 12S mitochondrial ribosomal RNA. This study corroborate the fact sex of guineafowl keet can be easily identified at genomic DNA level and they can be characterized taxonomically using the 12SrRNA mitochondrial genes.
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