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논문 기본 정보

자료유형
학술저널
저자정보
Young-Ho Cho (Korea Advanced Institute of Science and Technology) Tae Hee Lee (Eulji University) Jae-Eul Shim (Korea Advanced Institute of Science and Technology) Jiyoon Bu (University of Wisconsin-Madison)
저널정보
나노기술연구협의회 Nano Convergence Nano Convergence Vol.6 No.39
발행연도
2019.1
수록면
1 - 8 (8page)

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초록· 키워드

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Circulating tumor cells (CTCs) are receiving a great amount of scientific interest as a diagnostic biomarker for various types of cancer. Despite the recent progress in the development of highly sensitive CTC isolation devices, post-capture analysis of CTCs is still hindered by technical challenges associated with their rarity. Herein, we present a multi-modal CTC screening platform which is capable to analyze CTCs and CTC-derived extracellular vesicles (EVs), simultaneously from a single sample. Cytochalasin B (CB) treatment promotes cells to release large number of EVs from their surface, as demonstrated by CB-treated cells (5 µg/mL for 3 h) secreting 3.5-fold more EVs, compared to the non-treated cells. CB further generates 1.7-fold more EVs from the cells captured on our CTC filtration device (the fabric filter), compared to those from the cell culture flasks, owing to its multiple pore structure design which reduces the non-specific binding of EVs. Both CB-treated cancer cells and CB-induced EVs are found to overexpress tumor-associated markers, demonstrating a potential for the development of CTC dual-screening platform. Collectively, the results presented in this study reveal that our multi-modal cancer screening platform can synergistically improve the reliability and efficacy of the current CTC analysis systems.

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