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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한백신학회 Clinical and Experimental Vaccine Research Clinical and Experimental Vaccine Research Vol.8 No.2
- 발행연도
- 2019.1
- 수록면
- 124 - 131 (8page)
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Purpose: Canine influenza virus (CIV), H3N2, carries potentiality for zoonotic transmission and genetic assortment which raises a concern on possible epidemics, and human threats in future. To manage possible threats, the development of rapid and effective methods of CIV vaccine production is required. The plant provides economical, safe, and robust production platform. We investigated whether hemagglutinin (HA) antigen from Korea-originated CIV could be produced in Nicotiana benthamiana and lettuce, Lactuca sativa by a DNA viral vector system. Materials and Methods: We used DNA sequences of the HA gene from Korean CIV strain influenza A/canine/Korea/S3001/2015 (H3N2) for cloning into a geminiviral expression vectors to express recombinant HA (rHA) antigen in the plant. Agrobacterium-mediated infiltration was performed to introduce HA-carrying vector into host plants cells. Laboratory-grown N. benthamiana, and grocery-purchased or hydroponically-grown lettuce plant leaves were used as host plants. Results: CIV rHA antigen was successfully expressed in host plant species both N. benthamiana and L. sativa by geminiviral vector. Both complex-glycosylated and basal-glycosylated form of rHA were produced in lettuce, depending on presence of endoplasmic reticulum (ER) retention signal. In terms of rHA expression level, canine HA (H3N2) showed preference to the native signal peptide than ER retention signal peptide in the tested geminiviral vector system. Conclusion: Grocery-purchased lettuce leaves could serve as an instant host system for the transient expression of influenza antigen at the time of emergency. The geminiviral vector was able to induce expression of complex-glycosylated and basal-glycosylated rHA in lettuce and tobacco.
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참고문헌 (35)
참고문헌 신청
Crawford PC / 2005 / Transmission of equine influenza virus to dogs / Science 310:482~485
Song D / 2008 / Transmission of avian influenza virus(H3N2)to dogs / Emerg Infect Dis 14:741~746
Center for Disease Control and Prevention / 2019 / Key facts about canine influenza (dog flu) / Center for Disease Control and Prevention
Song DS / 2011 / Interspecies transmission of the canine influenza H3N2 virus to domestic cats in South Korea, 2010 / J Gen Virol 92(Pt 10):2350~2355
Kim H / 2013 / Inter- and intraspecies transmission of canine influenza virus (H3N2) in dogs, cats, and ferrets / Influenza Other Respir Viruses 7:265~270
Song D / 2008 / Transmission of avian influenza virus(H3N2)to dogs / Emerg Infect Dis 14:741~746
Center for Disease Control and Prevention / 2019 / Key facts about canine influenza (dog flu) / Center for Disease Control and Prevention
Song DS / 2011 / Interspecies transmission of the canine influenza H3N2 virus to domestic cats in South Korea, 2010 / J Gen Virol 92(Pt 10):2350~2355
Kim H / 2013 / Inter- and intraspecies transmission of canine influenza virus (H3N2) in dogs, cats, and ferrets / Influenza Other Respir Viruses 7:265~270
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