Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent <italic>Escherichia Coli</italic> from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon

Marbou Wiliane J. T.; Jain Priyanka; Samajpati Sriparna; Halder Gourab; Mukhopadhyay Asish K.; Dutta Shanta; Kuete Victor

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저자정보: Marbou Wiliane J. T. (University of Dschang) Jain Priyanka (Indian Council of Medical Research-National Institute of Cholera and Enteric Diseases) Samajpati Sriparna (Indian Council of Medical Research-National Institute of Cholera and Enteric Diseases) Halder Gourab (Indian Council of Medical Research-National Institute of Cholera and Enteric Diseases) Mukhopadhyay Asish K. (Indian Council of Medical Research-National Institute of Cholera and Enteric Diseases) Dutta Shanta (Indian Council of Medical Research-National Institute of Cholera and Enteric Diseases) Kuete Victor (University of Dschang)

저널정보: 질병관리본부 Osong Public Health and Research Persptectives Osong Public Health and Research Persptectives Vol.11 No.4

발행연도: 2020.1

수록면: 216 - 230 (15page)

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Objectives This study aimed to identify virulent and antimicrobial resistant genes in fecal E. coli in Mbouda, Cameroon. Methods A total of 599 fecal samples were collected from patients with enteric infections who were ≥ 20 years old. E. coli was isolated on the MacConkey agar and virulent genes were detected by multiplex/simplex PCR. Isolates in which ≥ 1 virulent gene was detected were subjected to antibiotic susceptibility testing. The resulting resistant isolates were subjected to PCR, followed by sequencing for resistant genes detection. Results There were 119 enterovirulent E. coli identified, amongst which 47.05% were atypical enteropathogenic E. coli (EPEC), 36.97% enterotoxigenic E. coli, 10.08% Shiga toxin producing E. coli (STEC) and 5.88% were enteroinvasive E. coli (EIEC). The occurrence of the eae gene (47.06%) was higher compared with CVD432 (33.61%), aaic (13.45%), stx2 (10.08%) and stx1 (0.84%). High resistance rates were noted for ampicillin (94.64% EPEC, 91.67% STEC, 59.09% EAEC, and 57.14% EIEC) and sulfamethoxazole-trimethoprim (100% EPEC and 83.33% STEC, 81.82% EAEC and 71.43% EIEC). sul2 (71.43%), tetB (64.71%), tetA (59.94%) and blaTEM (52.10%) were detected. A double mutation (S83L; D87N) was seen in gyrA and a single mutation (S80I) was observed in parC.

#E. coli #virulence #antibiotics resistance

참고문헌 (54)

참고문헌 신청

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Marbou Wiliane J. T.

소속기관 University of Dschang

주요연구분야 의약학 > 의학 일반

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Jain Priyanka