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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 467 - 478 (12page)
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Background: Schwann cells (SCs) secrete neurotrophic factors and provide structural support and guidance during axonal regeneration. However, nearby nerves may be damaged to obtain primary SCs, and there is a lack of nervous tissue donors. We investigated the potential of Wharton’s Jelly-derived mesenchymal stem cells (WJ-MSCs) in differentiating into Schwann cell-like cells (WJ-SCLCs) as an alternative to SCs. We also examined whether implantation of WJ-SCLCs-laden acellular nerve grafts (ANGs) are effective in inducing functional recovery and nerve regeneration in an animal model of peripheral nerve injury. Methods: The differentiation of WJ-MSCs into WJ-SCLCs was determined by analyzing SC-specific markers. The secretion of neurotrophic factors was assessed by the Neuro Discovery antibody array. Neurite outgrowth and myelination of axons were found in a co-culture system involving motor neuron cell lines. The effects of ANGs on repairing sciatic nerves were evaluated using video gait angle test, isometric tetanic force analysis, and toluidine blue staining. Results: Compared with undifferentiated WJ-MSCs, WJ-SCLCs showed higher expression levels of SC-specific markers such as S100β, GFAP, KROX20, and NGFR. WJ-SCLCs also showed higher secreted amounts of brain-derived neurotrophic factor, glial cell-derived neurotrophic factor, and granulocyte-colony stimulating factor than did WJ-MSCs. WJ-SCLCs effectively promoted the outgrowth and myelination of neurites in motor neuron cells, and WJ-SCLCs laden ANGs significantly facilitated peripheral nerve regeneration in an animal model of sciatic nerve injury. Conclusion: WJ-MSCs were readily differentiated into WJ-SCLCs, which effectively promoted the regeneration of peripheral nerves. Transplantation of WJ-SCLCs with ANGs might be useful for assisting peripheral nerve regeneration.
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참고문헌 (60)
참고문헌 신청
He B / 2014 / Factors predicting sensory and motor recovery after the repair of upper limb peripheral nerve injuries / Neural Regen Res 9:661~672
Li R / 2014 / Peripheral nerve injuries treatment : a systematic review / Cell Biochem Biophys 68:449~454
Wang AYL / 2019 / Topical application of human Wharton’s jelly mesenchymal stem cells accelerates mouse sciatic nerve recovery and is associated with upregulated neurotrophic factor expression / Cell Transplant 28:1560~1572
Vanderhooft E / 2000 / Functional outcomes of nerve grafts for the upper and lower extremities / Hand Clin 16:93~104
Fan L / 2014 / Schwann-like cells seeded in acellular nerve grafts improve nerve regeneration / BMC Musculoskelet Disord 15:165
Li R / 2014 / Peripheral nerve injuries treatment : a systematic review / Cell Biochem Biophys 68:449~454
Wang AYL / 2019 / Topical application of human Wharton’s jelly mesenchymal stem cells accelerates mouse sciatic nerve recovery and is associated with upregulated neurotrophic factor expression / Cell Transplant 28:1560~1572
Vanderhooft E / 2000 / Functional outcomes of nerve grafts for the upper and lower extremities / Hand Clin 16:93~104
Fan L / 2014 / Schwann-like cells seeded in acellular nerve grafts improve nerve regeneration / BMC Musculoskelet Disord 15:165
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