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논문 기본 정보

자료유형
학술저널
저자정보
Lee Jong-Yoon (The Catholic University of Korea) Kim Young-Hoon (The Catholic University of Korea) Kim Boo-Young (Ewha Womans University Seoul Hospital) Jang Dae-Hyun (Incheon St. Mary’s Hospital The Catholic University of Korea) Choi Sung-Wook (Department of Biotechnology The Catholic University of Korea) Joen So-Hyun (Incheon St. Mary’s Hospital The Catholic University of Korea) Kim Hyungyun (Incheon St. Mary’s Hospital The Catholic University of Korea) Lee Sang-Uk (Incheon St. Mary’s Hospital The Catholic University of Korea)
저널정보
한국조직공학과 재생의학회 조직공학과 재생의학 조직공학과 재생의학 제18권 제3호
발행연도
2021.1
수록면
453 - 465 (13page)

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초록· 키워드

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BACKGROUND: Autologous nerve grafts are the gold standard treatment for peripheral nerve injury treatment. However, this procedure cannot avoid sacrificing other nerves as a major limitation. The aim of the present study was to evaluate the potential of olfactory ensheathing cells (OECs) embedded in a nerve conduit. METHODS: A 10-mm segment of the sciatic nerve was resected in 21 rats, and the nerve injury was repaired with one of the following (n = 7 per group): autologous nerve graft, poly (ε-caprolactone) (PCL) conduit and OECs, and PCL conduit only. The consequent effect on nerve regeneration was measured based on the nerve conduction velocity (NCV), amplitude of the compound muscle action potential (ACMAP), wet muscle weight, histomorphometric analysis, and nerve density quantification. RESULTS: Histomorphometric analysis revealed nerve regeneration and angiogenesis in all groups. However, there were significant differences (p < 0.05) in the ACMAP nerve regeneration rate of the gastrocnemius and tibialis anterior muscles between the autologous graft (37.9 ± 14.3% and 39.1% ± 20.4%) and PCL only (17.8 ± 8.6% and 13.6 ± 5.8%) groups, and between the PCL only and PCL + OECs (46.3 ± 20.0% and 34.5 ± 14.6%) groups, with no differences between the autologous nerve and PCL + OEC groups (p > 0.05). No significant results in NCV, wet muscle weight, and nerve density quantification were observed among the 3 groups. CONCLUSION: A PCL conduit with OECs enhances the regeneration of injured peripheral nerves, offering a good alternative to autologous nerve grafts.

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