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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 641 - 650 (10page)
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초록· 키워드
오류제보하기
Background: Intestinal organoids have evolved as potential molecular tools that could be used to study host-microbiome interactions, nutrient uptake, and drug screening. Gut epithelial barrier functions play a crucial role in health and diseases, especially in autoimmune diseases, such as inflammatory bowel diseases (IBDs), because they disrupt the epithelial mucosa and impair barrier function. Methods: In this study, we generated an in vitro IBD model based on dextran sodium sulfate (DSS) and intestinal organoids that could potentially be used to assess barrier integrity. Intestinal organoids were long-term cultivated and characterized with several specific markers, and the key functionality of paracellular permeability was determined using FITC-dextran 4 kDa. Intestinal organoids that had been treated with 2 µM DSS for 3 h were developed and the intestinal epithelial barrier function was sequentially evaluated. Results: The results indicated that the paracellular permeability represented epithelial characteristics and their barrier function had declined when they were exposed to FITC-dextran 4 kDa after DSS treatment. In addition, we analyzed the endogenous mRNA expression of pro-inflammatory cytokines and their downstream effector genes. The results demonstrated that the inflammatory cytokines genes significantly increased in inflamed organoids compared to the control, leading to epithelial barrier damage and dysfunction. Conclusion: The collective results showed that in vitro 3D organoids mimic in vivo tissue topology and functionality with minor limitations, and hence are helpful for testing disease models.
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참고문헌 (40)
참고문헌 신청
Chaithanya Chelakkot / 2018 / Mechanisms regulating intestinal barrier integrity and its pathological implications / Experimental and Molecular Medicine 50:1~9
Deuring JJ / 2013 / The cell biology of the intestinal epithelium and its relation to inflammatory bowel disease / Int J Biochem Cell Biol 45:798~806
Kraiczy J / 2019 / Intestinal epithelial organoids as tools to study epigenetics in gut health and disease / Stem Cells Int 2019:7242415
Lancaster MA / 2014 / Organogenesis in a dish : modeling development and disease using organoid technologies / Science 345:1247125
Sato T / 2009 / Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche / Nature 459:262~265
Deuring JJ / 2013 / The cell biology of the intestinal epithelium and its relation to inflammatory bowel disease / Int J Biochem Cell Biol 45:798~806
Kraiczy J / 2019 / Intestinal epithelial organoids as tools to study epigenetics in gut health and disease / Stem Cells Int 2019:7242415
Lancaster MA / 2014 / Organogenesis in a dish : modeling development and disease using organoid technologies / Science 345:1247125
Sato T / 2009 / Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche / Nature 459:262~265
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