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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 187 - 194 (8page)
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Whole blood is one of the most widely utilized human samples in biological research and is useful for analyzing the mechanisms of diverse bio-molecular phenomena. However, owing to its fluidic properties, whole blood is relatively unstable in the frozen state compared to other samples. Because RNA is structurally unstable, sample damage can severely affect RNA quality, thereby reducing its usability. This study aimed to assess the quality of RNA which was prepared from blood stored at different temperatures and times prior to freezing, as well as the effect of long-term freezing. The quality of the RNA derived from different blood samples was assessed by measuring the RNA integrity number (RIN) and RNA sequencing to identify differentially expressed genes (DEGs, |fold-change (FC)|> 1.5, p < 0.05, false discovery rate (FDR) < 0.05) between the differently prepared RNA samples. We found that improper sample handling critically influenced both RNA quality and gene expression patterns. By suggesting the consequences, this study emphasizes the importance of sample management to obtain reliable downstream application outcomes.
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참고문헌 (16)
참고문헌 신청
Jeong, K. S. / 2019 / Cohort profi le: beyond birth cohort study–The Korean CHildren’s ENvironmental health Study (Ko-CHENS) / Environ. Res 172:358~366
Elliott, P. / 2008 / The UK Biobank sample handling and storage protocol for the collection, processing and archiving of human blood and urine / Int. J. Epidemiol 37(2):234~244
Shabihkhani, M. / 2014 / The procurement, storage, and quality assurance of frozen blood and tissue biospecimens in pathology, biorepository, and biobank settings / Clin. Biochem 47(4?5):258~266
Opitz, L. / 2010 / Impact of RNA degradation on gene expression profi ling / BMC Biol 3(1):36
Shen, Y. / 2018 / Impact of RNA integrity and blood sample storage conditions on the gene expression analysis / OncoTargets Ther 11:3573
Elliott, P. / 2008 / The UK Biobank sample handling and storage protocol for the collection, processing and archiving of human blood and urine / Int. J. Epidemiol 37(2):234~244
Shabihkhani, M. / 2014 / The procurement, storage, and quality assurance of frozen blood and tissue biospecimens in pathology, biorepository, and biobank settings / Clin. Biochem 47(4?5):258~266
Opitz, L. / 2010 / Impact of RNA degradation on gene expression profi ling / BMC Biol 3(1):36
Shen, Y. / 2018 / Impact of RNA integrity and blood sample storage conditions on the gene expression analysis / OncoTargets Ther 11:3573
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