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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 127 - 132 (6page)
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Degeneration of motor neurons and skeletal muscles or the collapse of neuromuscular junctions (NMJs) causes progressive motility disturbances in many neuromuscular diseases. Although various microdevices for the co-culture of skeletal muscle myotubes and motor neurons have been developed to investigate neuromuscular diseases in vitro, it remains difficult to isolate single myotubes and motor neurons from the device for single-cell analyses, such as gene expression analysis. Here, we developed open chamber-coculture microdevices that contain cell culture chambers with narrow widths. Given the small chamber width (0.2 mm), the device significantly prevented the overlap among myotubes within the chamber. The percentage of non-overlapping was 95.6 ± 7.7% for the 0.2-mmwidth chamber and 11.8 ± 6.4% for the 7-mm-width chamber as a control. In addition, the device with the 0.2-mm chamber promoted myotube maturation, as indicated by the longer widths and lengths of the myotubes relative to those in the control chamber. Single C2C12 myotubes and human induced pluripotent stem cell (hiPSC)-derived motor neurons were successfully collected from the device with the 0.2-mm chamber using a micromanipulator equipped with a glass capillary. Furthermore, myotubes and hiPSC-derived motor neurons were co-cultured in the device with the 0.2- mm chamber, and the formation of NMJs were observed. Thus, the developed device is a useful tool for performing single-cell analysis for studying neuromuscular diseases in vitro.
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참고문헌 (20)
참고문헌 신청
Witzemann, V. / 2006 / Development of the neuromuscular junction / Cell Tissue Res 326:263~271
Darabid, H. / 2014 / Neuromuscular synaptogenesis: coordinating partners with multiple functions / Nat. Rev. Neurosci 15:703~718
Fischer, L. R. / 2004 / Amyotrophic lateral sclerosis is a distal axonopathy: evidence in mice and man / Exp. Neurol 185:232~240
Wong, M. / 2010 / Skeletal muscle-restricted expression of human SOD1 causes motor neuron degeneration in transgenic mice / Hum. Mol. Genet 19:2284~2302
Robitaille, R. / 1993 / Functional colocalization of calcium and calcium-gated potassium channels in control of transmitter release / Neuron 11:645~655
Darabid, H. / 2014 / Neuromuscular synaptogenesis: coordinating partners with multiple functions / Nat. Rev. Neurosci 15:703~718
Fischer, L. R. / 2004 / Amyotrophic lateral sclerosis is a distal axonopathy: evidence in mice and man / Exp. Neurol 185:232~240
Wong, M. / 2010 / Skeletal muscle-restricted expression of human SOD1 causes motor neuron degeneration in transgenic mice / Hum. Mol. Genet 19:2284~2302
Robitaille, R. / 1993 / Functional colocalization of calcium and calcium-gated potassium channels in control of transmitter release / Neuron 11:645~655
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