지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 90 - 99 (10page)
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초록· 키워드
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Purpose: To investigate potential target genes associated with the diabetic condition in mouse cavernous endothelial cells (MCECs) for the treatment of diabetes-induced erectile dysfunction (ED). Materials and Methods: Mouse cavernous tissue was embedded into Matrigel, and sprouted cells were subcultivated for other studies. To mimic diabetic conditions, MCECs were exposed to normal-glucose (NG, 5 mmoL) or high-glucose (HG, 30 mmoL) conditions for 72 hours. An RNA-sequencing assay was performed to evaluate gene expression profiling, and RT-PCR was used to validate the sequencing data. Results: We isolated MCECs exposed to the two glucose conditions. MCECs showed well-organized tubes and dynamic migration in the NG condition, whereas tube formation and migration were significantly decreased in the HG condition. RNA-sequencing analysis showed that MCECs had different gene profiles in the NG and HG conditions. Among the significantly changed genes, which we classified into 14 major gene categories, we identified that aging-related (9.22%) and angiogenesis-related (9.06%) genes were changed the most. Thirteen genes from the two gene categories showed consistent changes on the RNA-sequencing assay, and these findings were validated by RT-PCR. Conclusions: Our gene expression profiling studies showed that Cyp1a1, Gclm, Igfbp5, Nqo1, Il6, Cxcl5, Olr1, Ctgf, Hbegf, Serpine1, Cyr61, Angptl4, and Loxl2 may play a critical role in diabetes-induced ED through aging and angiogenesis signaling. Additional research is necessary to help us understand the potential mechanisms by which these genes influence diabetes-induced ED.
목차
등록된 정보가 없습니다.
참고문헌 (15)
참고문헌 신청
Musicki B / 2007 / Endothelial dysfunction in diabetic erectile dysfunction / Int J Impot Res 19:129~138
Andrade E / 2008 / cDNA microarray analysis of differentially expressed genes in penile tissue after treatment with tadalafil / BJU Int 101:508~512
Yin GN / 2018 / Pericyte-derived Dickkopf2 regenerates damaged penile neurovasculature through an angiopoietin-1-Tie2 pathway / Diabetes 67:1149~1161
Castela  / 2016 / Molecular mechanisms associated with diabetic endothelial-erectile dysfunction / Nat Rev Urol 13:266~274
Katz PS / 2011 / Coronary arterioles in type 2 diabetic(db/db)mice undergo a distinct pattern of remodeling associated with decreased vessel stiffness / Basic Res Cardiol 106:1123~1134
Andrade E / 2008 / cDNA microarray analysis of differentially expressed genes in penile tissue after treatment with tadalafil / BJU Int 101:508~512
Yin GN / 2018 / Pericyte-derived Dickkopf2 regenerates damaged penile neurovasculature through an angiopoietin-1-Tie2 pathway / Diabetes 67:1149~1161
Castela  / 2016 / Molecular mechanisms associated with diabetic endothelial-erectile dysfunction / Nat Rev Urol 13:266~274
Katz PS / 2011 / Coronary arterioles in type 2 diabetic(db/db)mice undergo a distinct pattern of remodeling associated with decreased vessel stiffness / Basic Res Cardiol 106:1123~1134
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