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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 303 - 311 (9page)
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Purpose: Proprotein convertase subtilisin/kexin type 4 (PCSK4, a 54-kDa protease) is expressed in the plasma membrane of the acrosome in human spermatozoa. It plays a critical role in penetrating the zona pellucida. Synthesis of human anti-PCSK4 might be important for novel male immunocontraception. Materials and Methods: We used semen from adult males as the source of antigen (acrosomal PCSK4). Isolation and antigen characterization were done by immunohistochemistry followed by electrophoresis. Purification of PCSK4 was done by the electroelution method, followed by immunization with an animal model (Oryctolagus cuniculus). Antibody was collected, purified, and tested by using Western blot and dot blot. Antibody in vitro potential testing was performed on human spermatozoa by laser scanning microscopy with rhodamine stain under a light microscope and on rat spermatozoa. Results: Human anti-PCSK4 bound with PCSK4 on the head of human spermatozoa and could interfere with rat spermatozoa activity to penetrate the oocyte. Conclusions: The result of this study can be used as a basis to develop new immunocontraceptives targeted towards males. This study shows that antibodies from induction of PCSK4 from spermatozoa may hinder fertilization.
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등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
Page ST / 2008 / Advances in male contraception / Endocr Rev 29:465~493
Gupta SK / 2011 / Zona pellucida-based contraceptive vaccines for human and animal utility / J Reprod Immunol 88:240~246
Naz RK / 2009 / Immune infertility / Springer :197~207
Naz RK / 2004 / Human synthetic peptide vaccine for contraception targeting sperm / Arch Androl 50:113~119
Gyamera-Acheampong C / 2009 / The physiology and biochemistry of the fertility enzyme Proprotein Convertase Subtilisin/Kexin type 4
Gupta SK / 2011 / Zona pellucida-based contraceptive vaccines for human and animal utility / J Reprod Immunol 88:240~246
Naz RK / 2009 / Immune infertility / Springer :197~207
Naz RK / 2004 / Human synthetic peptide vaccine for contraception targeting sperm / Arch Androl 50:113~119
Gyamera-Acheampong C / 2009 / The physiology and biochemistry of the fertility enzyme Proprotein Convertase Subtilisin/Kexin type 4
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