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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 513 - 522 (10page)
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Background Sulfide is a well-known toxicant widely distributed in the culture environment. As a representative burrowing benthic bivalve, the razor clam Sinonovacula constricta is highly sulfide tolerant. Mitochondrial sulfide oxidation is an important way for sulfide detoxification, where sulfur dioxygenase (SDO) is the second key enzyme. Objective To investigate the mechanism of sulfide tolerance in S. constricta, the molecular characterization of its SDO (designated as ScSDO) was studied. Methods The cDNA sequence of ScSDO was cloned by RACE technique. The response of ScSDO in gills and livers of S. constricta was investigated during sulfide exposure (50, 150, and 300 μM sulfide) for 0, 3, 6, 12, 24, 48, 72, and 96 h by qRT-PCR. Moreover, the temporal expression of ScSDO protein in S. constricta gills after exposure to 150 μM sulfide was detected by Western blot. The subcellular location of ScSDO was identified by TargetP 1.1 prediction and Western Blot analysis. Results The full-length cDNA of ScSDO was 2914 bp, encoding a protein of 304 amino acids. The deduced ScSDO protein was highly conserved, containing the signature HXHXDH motif of the metallo-β-lactamase superfamily and two metal-binding sites, of which metal-binding site I is known to be the catalytically active center. Subcellular localization confirmed that ScSDO was located only in the mitochondria. Responding to the sulfide exposure, distinct time-dependent increases in ScSDO expression were detected at both mRNA and protein levels. Moreover, the gills exhibited a higher ScSDO expression level than the livers. Conclusions All of our results suggest that ScSDO plays an important role in mitochondrial sulfide oxidation during sulfide stress, making S. constricta highly sulfide tolerant. In addition, as a respiratory tissue, the gills play a more critical role in sulfide detoxification.
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참고문헌 (51)
참고문헌 신청
Afonso EG / 2002 / Blood parameters and metabolites in the teleost fsh Colossoma macropomum exposed to sulfde or hypoxia / Comp Biochem Physiol C 133:375~382
Afonso EG / 2004 / Physiological responses to sulfde toxicity by the air-breathing catfsh, hoplosternum littorale(siluriformes, callichthyidae) / Comp Biochem Physiol C 139:251~257
Arp AJ / 1992 / Burrow environment and coelomic fuid characteristics of the echiurian worm Urechis caupo from populations at three sites in northern California / Mar Biol 113:613~623
Bagarinao T / 1992 / Sulfde as an environmental factor and toxicant : tolerance and adaptations in aquatic organisms / Aquat Toxicol 24:21~62
Bagarinao T / 1990 / Oxidative detoxifcation of sulfde by mitochondria of the California killifsh Fundulus parvipinnis and the speckled sanddab Citharichthys sitgmaeus / J Comp Physiol B 160:519~527
Afonso EG / 2004 / Physiological responses to sulfde toxicity by the air-breathing catfsh, hoplosternum littorale(siluriformes, callichthyidae) / Comp Biochem Physiol C 139:251~257
Arp AJ / 1992 / Burrow environment and coelomic fuid characteristics of the echiurian worm Urechis caupo from populations at three sites in northern California / Mar Biol 113:613~623
Bagarinao T / 1992 / Sulfde as an environmental factor and toxicant : tolerance and adaptations in aquatic organisms / Aquat Toxicol 24:21~62
Bagarinao T / 1990 / Oxidative detoxifcation of sulfde by mitochondria of the California killifsh Fundulus parvipinnis and the speckled sanddab Citharichthys sitgmaeus / J Comp Physiol B 160:519~527
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