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자료유형
학술저널
저자정보
Qian Wang (Tianjin Academy of Agricultural Sciences) Yingxia Yang (Tianjin Academy of Agricultural Sciences) Guoqing Lu (Tianjin Academy of Agricultural Sciences) Xianjun Sun (Chinese Academy of Agricultural Sciences) Youren Feng (Tianjin Academy of Agricultural Sciences) Shuangyong Yan (Tianjin Academy of Agricultural Sciences) Huiyuan Zhang (Chinese Academy of Agricultural Sciences) Qiyan Jiang (Chinese Academy of Agricultural Sciences) Hui Zhang (Chinese Academy of Agricultural Sciences) Zheng Hu (Chinese Academy of Agricultural Sciences) Rui Chen (Tianjin Academy of Agricultural Sciences Tianjin 300381 P.R. China)
저널정보
한국유전학회 Genes & Genomics Genes & Genomics Vol.42 No.11
발행연도
2020.1
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1,239 - 1,249 (11page)

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Background Salinity stress, as the key limiting factor for agricultural productivity, can activate a series of molecular responses and alter gene expression in plants. Endogenous regulatory small RNAs, such as microRNAs (miRNAs) and phased siRNAs (phasiRNAs), play crucial roles during stress adaptation and prevent the injury from environmental circumstances. Objective To identify long-term salt stress responsive miRNAs and phasiRNAs as well as their associated genes and pathways in soybean roots. Methods Small RNA and degradome sequencing strategies were applied to genome widely investigate miRNAs and phasiRNAs in soybean roots under control and long-term salt stress conditions. Results In this study, stringent bioinformatic analysis led to the identifcation of 253 conserved and 38 novel miRNA candidates. Results of expression profling, target and endogenous target mimics predictions provided valuable clues to their functional roles. Furthermore, 156 genes were identifed to be capable of generating 21 nt and 24 nt phasiRNAs, in which 37 candidates were confrmed by degradome data for miRNA-directed cleavage. Approximately 90% of these phasiRNA loci were protein coding genes. And GO enrichment analysis pointed to “signal transduction” and “ADP binding” entries and refected the functional roles of identifed phasiRNA genes. Conclusion Taken together, our fndings extended the knowledge of salt responsive miRNAs and phasiRNAs in soybean roots, and provided valuable information for a better understanding of the regulatory events caused by small RNAs underlying plant adaptations to long-term salt stress.

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