Genetic localization of the SPC gene controlling pod coiling direction in Medicago truncatula

Xiaocheng Yu; Qiulin Qin; Xia Wu; Dandan Li; Shengming Yang

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자료유형: 학술저널

저자정보: Xiaocheng Yu (University of Kentucky) Qiulin Qin (University of Kentucky) Xia Wu (University of Kentucky) Dandan Li (University of Kentucky) Shengming Yang (University of Kentucky)

저널정보: 한국유전학회 Genes & Genomics Genes & Genomics Vol.42 No.7

발행연도: 2020.1

수록면: 735 - 742 (8page)

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Background Handedness in plants introduced by helical growth of organs is frequently observed, and it has fascinated plant scientists for decades. However, the genetic control of natural handedness has not been revealed. In the model legume Medicago truncatula, pods can be coiled in a clockwise or anti-clockwise manner, providing a model for genetic analysis of plant handedness. Objective We aimed to localize the Sense of Pod Coiling (SPC) gene controlling pod coiling direction in M. truncatula. Methods Linkage analysis was used with a biparental population for fine mapping of the SPC gene. The genome sequence of M. truncatula Mt4.0 was used for marker identification and physical mapping. Single nucleotide polymorphisms (SNPs) between the parental lines were converted to CAPS (cleaved amplified polymorphic sequences) markers. Genetic map was constructed using the software JoinMap version 3.0. Gene predication and annotation provided by the M. truncatula genome database (http://www.medic agoge nome.org) was confirmed with the programs of FGENESH and Pfam 32.0, respectively. Quantitative reverse transcription PCR (qRT-PCR) was used to analyze the relative expression levels of candidate genes. Results The genetic analysis indicated that the anti-clockwise coiling is dominant to clockwise and is controlled by the single gene, SPC. The SPC gene was delimited to a 250 kb-region on Chromosome 7. Total of 15 protein-coding genes were identified in the SPC locus through gene annotation and sequence analysis. Of those, two genes, potentially encoding a receptor-like kinase and a vacuolar cation/proton exchanger respectively, were selected as candidates for the SPC gene. Conclusions The result presented here lay a foundation for gene cloning of SPC, which will help us to understand the molecular mechanisms underlying helical growth in plant organs.

#Plant handedness · M. truncatula · Pod coiling · SPC · Genetic mapping

참고문헌 (43)

참고문헌 신청

Bassil E / 2012 / Cellular ion homeostasis : emerging roles of intracellular NHX Na+/H + antiporters in plant growth and development / J Exp Bot 63(16):5727~5740

Bateman A / 2004 / The Pfam protein families database / Nucleic Acids Res 32(Database issue):D138~D141

Burk DH / 2002 / Alteration of oriented deposition of cellulose microfibrils by mutation of a katanin-like microtubulesevering protein / Plant Cell 14(9):2145~2160

Buschmann H / 2020 / Handedness in plant cell expansion : a mutant perspective on helical growth / New Phytol 225(1):53~69

Chevalier D / 2005 / STRUBBELIG defines a receptor kinasemediated signaling pathway regulating organ development in Arabidopsis / Proc Natl Acad Sci USA 102(25):9074~9079

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