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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 167 - 174 (8page)
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Beta-transducin repeat containing protein 1 (β-TrCP1) is a versatile F-box protein that is responsible for substrate recognition of SCFβ- TrCP1 ubiquitin ligase. In human cells, two major alternatively spliced isoforms (b and f) of β-TrCP1 were found. Recently, we identified that CENP-W interacts with the β-TrCP1 and regulates the cellular distribution of β-TrCP1. In this study, we examined whether CENP-W, a new kinetochore component, may differentially regulate the two major isoforms of human β-TrCP1 (b and f), especially in the cytoplasmic-nuclear shuttling of β-TrCP1. An in vivo binding assay was performed to examine whether CENP-W binds differently to the two isoforms of β-TrCP1. EGFP-conjugated β-TrCP1 isoforms were co-transfected with NLS-defective mutant CENP-W and their cellular distribution were observed using a fluorescence microscopy. Although CENP-W interacts with both b and f isoforms, it has a greater affinity for the b isoform rather than f isoform. Moreover, CENP-W effectively regulates the nuclear-cytoplasmic shuttling of these two β-TrCP1 isoforms, but with a slight preference towards the b isoform. The Elongin C-binding motif existing in the b isoform may be involved in their specific association. CENP-W showed a higher affinity toward the β-TrCP1 b isoform, and translocated isoform b more efficiently than isoform f, which may allow a fine regulation of of β-TrCP1 in the cells.
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참고문헌 (26)
참고문헌 신청
Ang XL / 2005 / SCF-mediated protein degradation and cell cycle control / Oncogene 24:2860~2870
Besnard-Guerin C / 2004 / HIV-1 Vpu sequesters betatransducin repeat-containing protein(betaTrCP)in the cytoplasm and provokes the accumulation of beta-catenin and other SCFbetaTrCP substrates / J Biol Chem 279:788~795
Cheon Y / 2016 / Centromere protein W interacts with beta-transducin repeat-containing protein 1 and modulates its subcellular localization / FEBS Lett 590:4441~4452
Chun Y / 2011 / New centromeric component CENP-W is an RNA-associated nuclear matrix protein that interacts with nucleophosmin/B23 protein / J Biol Chem 286:42758~42769
Chun Y / 2013 / CSN5/JAB1 interacts with the centromeric components CENP-T and CENP-W and regulates their proteasome-mediated degradation / J Biol Chem 288:27208~27219
Besnard-Guerin C / 2004 / HIV-1 Vpu sequesters betatransducin repeat-containing protein(betaTrCP)in the cytoplasm and provokes the accumulation of beta-catenin and other SCFbetaTrCP substrates / J Biol Chem 279:788~795
Cheon Y / 2016 / Centromere protein W interacts with beta-transducin repeat-containing protein 1 and modulates its subcellular localization / FEBS Lett 590:4441~4452
Chun Y / 2011 / New centromeric component CENP-W is an RNA-associated nuclear matrix protein that interacts with nucleophosmin/B23 protein / J Biol Chem 286:42758~42769
Chun Y / 2013 / CSN5/JAB1 interacts with the centromeric components CENP-T and CENP-W and regulates their proteasome-mediated degradation / J Biol Chem 288:27208~27219
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