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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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Background: The purpose of this study was to investigate the anti-oral microbial activity and anti-inflammatory effects of rosmarinic acid (RA) in lipopolysaccharide (LPS)-stimulated MC3T3-E1 osteoblastic cells on a titanium (Ti) surface during osseointegration, and to confirm the possibility of using RA as a safe natural substance for the control of peri-implantitis (PI) in Ti-based dental implants. Methods: A disk diffusion test was conducted to confirm the antimicrobial activity of RA against oral microorganisms. In order to confirm the anti-inflammatory effects of RA, inflammatory conditions were induced with 100 ng/ml of LPS in MC3T3-E1 osteoblastic cells on the Ti surface treated with or without 14μg/ml of RA. The production of nitric oxide (NO) and prostaglandin E2 (PGE2) in LPS-stimulated MC3T3-E1 osteoblastic cells on the Ti surface was confirmed using an NO assay kit and PGE2 enzyme-linked immunosorbent assay kit. Reverse transcription polymerase chain reaction and western blot analysis were performed to confirm the expression of interleukin (IL)-1β and tumor necrosis factor (TNF)-α in total RNA and protein. Results: RA showed weak antimicrobial effects against Streptococcus mutans and Escherichia coli, but no antimicrobial activity against the bacteria Aggregatibacter actinomycetemcomitans and the fungus Candida albicans. RA reduced the production of pro-inflammatory mediators, NO and PGE2, and proinflammatory cytokines, TNF-α and IL-1β, in LPS-stimulated MC3T3-E1 osteoblastic cells on the Ti surface at the protein and mRNA levels. Conclusion: RA not only has anti-oral microbial activity, but also anti-inflammatory effects in LPS-stimulated MC3T3-E1 osteoblasts on the Ti surface, therefore, it can be used as a safe functional substance derived from plants for the prevention and control of PI for successful Ti-based implants.
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참고문헌 (29)
참고문헌 신청
Jeong SJ / 2016 / Effect of thymosin beta4 on the differentiation and mineralization of MC3T3-E1 cell on a titanium surface / J Nanosci Nanotechnol 16:1979~1983
Choi BD / 2016 / Secretory leukocyte protease inhibitor promotes differentiation and mineralization of MC3T3-E1 preosteoblasts on a titanium surface / Mol Med Rep 14:1241~1246
Kokubo T / 2010 / Bioactive Ti metal and its alloys prepared by chemical treatments: state‐of‐the‐art and future trends / Adv Eng Mater 12:B579~B591
Khang D / 2012 / Role of subnano-, nano-and submicron-surface features on osteoblast differentiation of bone marrow mesenchymal stem cells / Biomaterials 33:5997~6007
Yamamoto H / 2002 / Antimicrobial activity of perilla seed polyphenols against oral pathogenic bacteria / Biosci Biotechnol Biochem 66:921~924
Choi BD / 2016 / Secretory leukocyte protease inhibitor promotes differentiation and mineralization of MC3T3-E1 preosteoblasts on a titanium surface / Mol Med Rep 14:1241~1246
Kokubo T / 2010 / Bioactive Ti metal and its alloys prepared by chemical treatments: state‐of‐the‐art and future trends / Adv Eng Mater 12:B579~B591
Khang D / 2012 / Role of subnano-, nano-and submicron-surface features on osteoblast differentiation of bone marrow mesenchymal stem cells / Biomaterials 33:5997~6007
Yamamoto H / 2002 / Antimicrobial activity of perilla seed polyphenols against oral pathogenic bacteria / Biosci Biotechnol Biochem 66:921~924
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