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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 100 - 111 (12page)
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초록· 키워드
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Objectives : The aim of experiment is to examine anti-inflammatory effect and anti-oxidant effect of Naesohwangryun-tang (NSHRT) in LPS-stimulated RAW264.7 macrophage cells. Methods : In the present study, The cell viability was performed by MTT assay. Nitric oxide (NO) production and prostaglandin E2 (PGE2) synthesis were performed by NO assay and ELISA KIT. The anti-oxidant effect was performed by DPPH and ABTS radical scavenging activity. The inhibitory effects of pro-inflammatory mediators and cytokines were confirmed by realtime PCR and western blotting. Results : NSHRT was no cytotoxicity at treated group. NO and PGE2 production were inhibited compared to the LPS treated group and also mRNA and protein expressions were significantly decreased compared to the LPS treated group. Conclusions : According to the above experiments, we confirmed that NSHRT has anti-inflammatory and anti-oxidant effects. It is suggested that NSHRT is potential ingredient of skin diseases.
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참고문헌 (31)
참고문헌 신청
Perer P / 2011 / Text of The Immune System / Lifescience :1~27
Kim IS / 2017 / Text of Essential Immunology / daihak :28~29
Smith JA / 2011 / Role of pro-inflammatory cytokines released from microglia in neurodegenerative diseases / Brain Research Bulletin 87(1):10~20
Hori M / 2001 / Upregulation of iNOS by COX-2 in muscularis resident macrophage of rat intestine stimulated with LPS / Journal of Physiology Gastrointestinal and Liver Physiology 280(5):930~938
Samuel JA / 1999 / Inhibitors of p38 and Extracellular Signal-Regulated Kinase Mitogen-Activated Protein Kinase Pathways Block Inducible Nitric Oxide Synthase and Tumor Necrosis Factor Accumulation in Murin Macrophages Stimulated with Lipopolysaccharide and Interferon-γ / The journal of Infectious Diseases 179(4):939~944
Kim IS / 2017 / Text of Essential Immunology / daihak :28~29
Smith JA / 2011 / Role of pro-inflammatory cytokines released from microglia in neurodegenerative diseases / Brain Research Bulletin 87(1):10~20
Hori M / 2001 / Upregulation of iNOS by COX-2 in muscularis resident macrophage of rat intestine stimulated with LPS / Journal of Physiology Gastrointestinal and Liver Physiology 280(5):930~938
Samuel JA / 1999 / Inhibitors of p38 and Extracellular Signal-Regulated Kinase Mitogen-Activated Protein Kinase Pathways Block Inducible Nitric Oxide Synthase and Tumor Necrosis Factor Accumulation in Murin Macrophages Stimulated with Lipopolysaccharide and Interferon-γ / The journal of Infectious Diseases 179(4):939~944
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