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Digestate or slurry produced from anaerobic digestion is mostly applied to crop lands for its disposal and recovering nutrients. However, minimizingnitrogen losses following field application of the digestate is important for maximizing the plant’s nitrogen uptake and reducing environmental concerns. This study was conducted to assess the effects of three different biogas digestate application techniques (sawdust mixed with digestate (SSD), the holeapplication method (HA), and digestate injected in the soil (SD)) on nitrate leaching potential in the soil. A pot laboratory experiment was conductedat room temperature of 25 ± 2 °C for 107 days. The experimental results showed that sawdust application method turned out to be appropriate forquick immobilization of surplus N in the form of microbial biomass N, reflecting its lower total nitrogen and NH4-N contents and low pH. The NH4-Nand total nitrogen fate in the soil fertilized with manure showed no statistically significant (p > 0.05) differences between the different methods appliedduring the incubation time under room temperature. In contrast, NO3-N concentration indicates significant reduction in sawdust treatment (p < 0.05)compared to the control and other application methods. However, the soil sawdust mixed with digestate was more effective than the other methods,because of the cumulative labile carbon contents of the amendment, which implies soil net N immobilization.
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