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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 26 - 36 (11page)
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초록· 키워드
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Background: Atopic dermatitis (AD) is characterized by chronic, relapsing skin inflammation (eczema) with itchy sensation. Keratinocytes, which are located at the outermost part of our body, are supposed to play important roles at the early phase of type 2 inflammation including AD pathogenesis. Objective: The purpose of this study was to evaluate whether keratinocytes-derived reactive oxygen species (ROS) could be produced by the allergens or non-allergens, and the keratinocytes- derived ROS could modulate a set of biomarkers for type 2 inflammation of the skin. Methods: Normal human epidermal keratinocytes (NHEKs) were treated with an allergen of house dust mites (HDM) or a non-allergen of compound 48/80 (C48/80). Then, biomarkers for type 2 inflammation of the skin including those for neurogenic inflammation were checked by reverse transcriptase-polymerase chain reaction and western immunoblot experiments. Results: HDM or C48/80 was found to upregulate expression levels of our tested biomarkers, including type 2 T helper-driving pathway (KLK5, PAR2, and NFκB), epithelial-cell-derived cytokines (thymic stromal lymphopoietin, interleukin [IL]-25, IL-33), and neurogenic inflammation (NGF, CGRP). The HDMor C-48/80-induced expression levels of the biomarkers could be blocked by an antioxidant treatment with 5 mM N-acetyl- cysteine. In contrast, pro-oxidant treatment with 1 mM H2O2 could upregulate expression levels of the tested biomarkers in NHEKs. Conclusion: Our results reveal that keratinocytes- derived ROS, irrespective to their origins from allergens or non-allergens, have a potential to induce type 2 inflammation of AD skin.
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참고문헌 (54)
참고문헌 신청
Cornelissen C / 2011 / Ultraviolet B radiation and reactive oxygen species modulate interleukin-31 expression in T lymphocytes, monocytes and dendritic cells / Br J Dermatol 165:966~975
Dobashi K / 2001 / Regulation of LPS induced IL-12 production by IFNgamma and IL-4 through intracellular glutathione status in human alveolar macrophages / Clin Exp Immunol 124:290~296
Murata Y / 2002 / The polarization of T(h)1/T(h)2 balance is dependent on the intracellular thiol redox status of macrophages due to the distinctive cytokine production / Int Immunol 14:201~212
Obata F / 2006 / Hydrogen peroxide increases interleukin-12 p40/p70 molecular ratio and induces Th2-predominant responses in mice / Scand J Immunol 63:125~130
Peterson JD / 1998 / Glutathione levels in antigen-presenting cells modulate Th1versus Th2 response patterns / Proc Natl Acad Sci U S A 95:3071~3076
Dobashi K / 2001 / Regulation of LPS induced IL-12 production by IFNgamma and IL-4 through intracellular glutathione status in human alveolar macrophages / Clin Exp Immunol 124:290~296
Murata Y / 2002 / The polarization of T(h)1/T(h)2 balance is dependent on the intracellular thiol redox status of macrophages due to the distinctive cytokine production / Int Immunol 14:201~212
Obata F / 2006 / Hydrogen peroxide increases interleukin-12 p40/p70 molecular ratio and induces Th2-predominant responses in mice / Scand J Immunol 63:125~130
Peterson JD / 1998 / Glutathione levels in antigen-presenting cells modulate Th1versus Th2 response patterns / Proc Natl Acad Sci U S A 95:3071~3076
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