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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 703 - 712 (10page)
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초록· 키워드
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The Glycogen synthase kinase 3 (GSK3)-like kinase BRASSINOSTEROID-INSENSITIVE2 (BIN2), a major negative regulator in the Brassinosteroids (BRs) signaling, is involved in a variety of plant signaling pathways by interacting with novel substrates and plays a major role in cellular, growth and developmental regulation. Despite BIN2 functional studies including BR signaling related proteasome-mediated BIN2 degradation, the molecular regulating mechanisms and it’s related the protein component for regulating BIN2 degradation has not been completely known. This study aimed to i) identify BIN2 protein and its interacting partner from HA-Immunoprecipitation (IP) of the BL-treated BIN2-HA and bin2-6D-HA lines, using liquid chromatography tandem mass spectrometry (LC?MS/MS) and matrix-assisted laser desorption/ionization time-of-fight/time-of-fight mass spectrometry (MALDI?TOF/TOF MS) and ii) characterize relationships between BIN2 and interacting partner proteins. We generated transgenic plants constitutively expressing BIN2-HA, bin2-6D (BIN2E264K)-HA, and BIN2KD (BIN2K69R)-HA construct. IP of the HA-tagged bin2-6D/BIN2 protein followed by mass spectrometry identifed F-box protein, BRASSINOSTEROID F-BOX 1 (BRF1) and BRF2 containing the LRR and FBD domain, as interacting proteins with BIN2. Validation in?vitro by yeast two-hybrid analyses further confrmed the interacting protein. These results, together with phylogeny and sequence alignments of BRF1 and BRF2 homologs multiple methods, suggest that f-box protein BRF1 and BRF2 play redundant or overlapping roles in regulating BIN2. It is likely that the BIN2 protein stability is controlled by BRF1 and BRF2.
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참고문헌 (35)
참고문헌 신청
Ali A / 2001 / Glycogen synthase kinase-3: properties, functions, and regulation / Chem Rev 101(8):2527~2540
Bijur GN / 2001 / Proapoptotic stimuli induce nuclear accumulation of glycogen synthase kinase-3 beta / J Biol Chem 276(40):37436~37442
Choe S / 2002 / Arabidopsis brassinosteroidinsensitive dwarf12 mutants are semidominant and defective in a glycogen synthase kinase 3 beta-like kinase / Plant Physiol 130(3):1506~1515
Clough SJ / 1998 / Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana / Plant J 16(6):735~743
Cox J / 2011 / Andromeda: a peptide search engine integrated in to the MaxQuant environment / J Proteome Res 10(4):1794~1805
Bijur GN / 2001 / Proapoptotic stimuli induce nuclear accumulation of glycogen synthase kinase-3 beta / J Biol Chem 276(40):37436~37442
Choe S / 2002 / Arabidopsis brassinosteroidinsensitive dwarf12 mutants are semidominant and defective in a glycogen synthase kinase 3 beta-like kinase / Plant Physiol 130(3):1506~1515
Clough SJ / 1998 / Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana / Plant J 16(6):735~743
Cox J / 2011 / Andromeda: a peptide search engine integrated in to the MaxQuant environment / J Proteome Res 10(4):1794~1805
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