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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 673 - 682 (10page)
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Squalene epoxidase (also called squalene monooxygenase) catalyses the conversion of squalene into 2,3-oxidosqualene by epoxidation and is regarded as the rate-limiting enzyme for sterol and saponin biosynthesis. However, the role of the squalene epoxidase gene in saponin biosynthesis in plants is not yet well understood. Here, we investigated the efects of overexpression of a Panax ginseng squalene epoxidase gene (PgSE1) on the production of phytosterols and ginsenoside saponins in ginseng adventitious roots. For the functional complementation test, the two squalene epoxidase sequences (PgSE1 and PgSE2) of P. ginseng were expressed in a yeast erg1?mutant (ergosterol auxotroph). The yeast mutant expressing PgSE1 or PgSE2 can restore growth on medium lacking ergosterol. Transgenic ginseng roots overexpressing the PgSE1 gene were constructed by Agrobacterium-mediated genetic transformation. The transgenic ginseng roots resulted in the enhanced production of both ginsenosides (ginsenoside Rg1, Re, Rf, Rc, Rb1, Rb2, and Rd) and phytosterols (campesterol, stigmasterol, and β-sitosterol). qPCR analysis revealed that overexpression of PgSE1 in transgenic ginseng roots clearly enhanced the expression of dammarenediol-II synthase (PgDDS) and cycloartenol synthase (PgPNX), which are key enzymes for ginse noside and phytosterol biosynthesis in P. ginseng. This result indicates that the P. ginseng squalene epoxidase gene (PgSE1) encodes an efcient enzyme responsible for not only phytosterol production but also ginsenoside production in P. ginseng.
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참고문헌 (24)
참고문헌 신청
Benfey PN / 1990 / The cauliflower mosaic virus 35S promoter:combinatorial regulation of transcription in plants / Science 250:959~966
Briskin DP / 2000 / Medicinal plants and phytomedicines. Linking plant biochemistry and physiology to human health / Plant Physiol 124:507~514
Choi YE / 2001 / Rapid and efficient Agrobacterium-mediated genetic transformation by plasmolyzing pretreatment of cotyledons in Panax ginseng / Plant Cell Rep 20:616~621
Coleman CI / 2003 / The effects of Panax ginseng on quality of life / J Clin Pharm Ther 28:5~15
Gundlach H / 1992 / Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures / Proc Natl Acad Sci USA 89:2389~2393
Briskin DP / 2000 / Medicinal plants and phytomedicines. Linking plant biochemistry and physiology to human health / Plant Physiol 124:507~514
Choi YE / 2001 / Rapid and efficient Agrobacterium-mediated genetic transformation by plasmolyzing pretreatment of cotyledons in Panax ginseng / Plant Cell Rep 20:616~621
Coleman CI / 2003 / The effects of Panax ginseng on quality of life / J Clin Pharm Ther 28:5~15
Gundlach H / 1992 / Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures / Proc Natl Acad Sci USA 89:2389~2393
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