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논문 기본 정보

자료유형
학술저널
저자정보
Wang Xiaochen (Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education TEDA College N) Xi Daoyi (Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education TEDA College N) Li Yuehua (Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education TEDA College N) Yan Junxiang (Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education TEDA College N) Zhang Jingyun (State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communica) Guo Xi (Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education TEDA College N) Cao Boyang (Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education TEDA College N)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제31권 제4호
발행연도
2021.1
수록면
520 - 528 (9page)

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Plesiomonas shigelloides, a member of the family Vibrionaceae, is a gram-negative, rod-shaped, facultative anaerobic bacterium with flagella. P. shigelloides has been isolated from such sources as freshwater, surface water, and many wild and domestic animals. P. shigelloides contains 102 Oantigens and 51 H-antigens. The diversity of O-antigen gene clusters is relatively poorly understood. In addition to O1 and O17 reported by other laboratories, and the 12 O serogroups (O2, O10, O12, O23, O25, O26, O32, O33, O34, O66, O75, and O76) reported previously by us, in the present study, nine new P. shigelloides serogroups (O8, O17, O18, O37, O38, O39, O44, O45, and O61) were sequenced and annotated. The genes for the O-antigens of these nine groups are clustered together in the chromosome between rep and aqpZ. Only O38 possesses the wzm and wzt genes for the synthesis and translocation of O-antigens via the ATP-binding cassette (ABC) transporter pathway; the other eight use the Wzx/Wzy pathway. Phylogenetic analysis using wzx and wzy showed that both genes are diversified. Among the nine new P. shigelloides serogroups, eight use wzx/wzy genes as targets. In addition, we developed an O-antigen-specific PCR assay to detect these nine distinct serogroups with no cross reactions among them.

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