지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.6
- 수록면
- 112 - 125 (14page)
이용수
초록· 키워드
오류제보하기
Bovine tuberculosis caused by Mycobacterium bovis is a major economic problem in several countries. Antibody responses are useful indicators of M. bovis infection of cattle. To overcome drawback of serological tests with low sensitivity, identification and characterization of multiple serodiagnostic antigens has been required. In this study, the antigens with strong antibody reactivity were searched using fractionation of M. bovis culture filtrate proteins and probing with sera from M. bovis-infected cattle. Twelve proteins which have not previously been described as serologic targets were identified and six proteins among them were expressed in Escherichia coli. The mycobacterial lipoarabinomannan (LAM) with strong seroreactivity in cattle was identified and purified. IgG and IgA responses against the newly identified proteins, the seroreactive proteins with strong antibody reactivity in human tuberculosis, and LAM were compared in M. bovis-infected and non-infected cattle as well as in field samples. In general, sensitivity of the tested antigens was higher in M. bovis-infected cattle than purified protein derivative (PPD) (+) field samples. Although a diverse reactivity and sensitivity according to the antigens were shown, the diagnostic utility of both IgA and IgG antibody to the antigens was similar in M. bovis-infected cattle but utility of IgG antibody was superior to that of IgA in field samples. The antigen with the highest diagnostic value was LAM in both the groups. Other antigens with considerable diagnostic utility were BCG_3488c, BCG_2330, Antigen 85, HspX, and Rv3593 when considered the sensitivity and area under the receiver characteristic curve (AUC) value. These antigens may be valuable candidates to be included in a cocktail test kit for bovine tuberculosis diagnosis.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Mucosal Antigen-targeting to Enhance Immune Response to Viral Antigens
한국미생물학회 학술대회논문집
2020 .10
-
Computational Design of SARS-CoV-2 Antigens for Protein Stability Enhancement
한국미생물학회 학술대회논문집
2022 .10
-
Development of Antigen Expression System for the Establish of Vaccine Efficacy Evaluation Method against Emerging Infectious Diseases
한국미생물학회 학술대회논문집
2022 .10
-
Intestinal Antigens Regulate IgE Homeostasis
한국미생물학회 학술대회논문집
2019 .04
-
Optimization of Yeast Surface-Displayed cDNA Library Screening for Low Abundance Targets
Journal of Microbiology and Biotechnology
2015 .01
-
Relationship between antigen by HPLC and antibody by virus neutralization test in Foot-and-Mouth-Disease virus serotype O and A
한국분석과학회 학술대회
2020 .06
-
Comparative Studies of Tuberculosis Immunoassay and Protective Ability according to the BCG Immunization Periods in Mouse Models
한국미생물학회 학술대회논문집
2022 .10
-
Correlation between antigen contents by HPLC and antibody value by VNT of foot-and-mouth disease serotype A vaccine
한국분석과학회 학술대회
2020 .06
-
Anti-Tuberculosis Activity of Pediococcus acidilactici Isolated from Young Radish Kimchi against Mycobacterium tuberculosis
Journal of Microbiology and Biotechnology
2021 .12
-
Construction and Characterization of a New Vaccine Candidate Using E. coli Antigens in Salmonella Ghost Delivery System
한국미생물학회 학술대회논문집
2015 .04
-
What Can Proteomics Tell Us about Tuberculosis?
Journal of Microbiology and Biotechnology
2015 .01
-
MALDI-MS analysis of proteins and lipids in bovine milk
한국분석과학회 학술대회
2019 .11
-
항결핵제 감수성 결핵에서의 내성 변화 추이
대한임상검사과학회지
2021 .01
-
Generation, Diversity Determination, and Application to Antibody Selection of a Human Naive Fab Library
Molecules and Cells
2017 .09
-
Immunogenicity of a DNA and Recombinant Protein Vaccine Combining LipL32 and Loa22 for Leptospirosis Using Chitosan as a Delivery System
Journal of Microbiology and Biotechnology
2015 .01
-
Fungal Allergen Propensity Study Confirmed Through Multiple Allergosorbent Chemiluminescent Assay
대한의진균학회지
2019 .01
-
대학생의 결핵에 관한 지식, 태도, 자기효능감이 결핵 예방행위에 미치는 영향
Journal of The Korean Data Analysis Society
2016 .01
-
In Vitro에서 히스타민 측정 시 ELISA법과 HPLC-MS 분석법의 비교
대한임상검사과학회지
2015 .01
-
대학생 잠복결핵 감염의 진단
대한임상검사과학회지
2016 .01
-
Measurement of Antibodies to Varicella-Zoster Virus Using a Virus-Free Fluorescent-Antibody-to-Membrane-Antigen (FAMA) Test
Journal of Microbiology and Biotechnology
2015 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2016-475-001637145