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논문 기본 정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제31권 제2호
- 발행연도
- 2021.1
- 수록면
- 233 - 239 (7page)
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Cyanobacteriochromes (CBCRs) are phytochrome-related photoreceptor proteins in cyanobacteria and cover a wide spectral range from ultraviolet to far-red. A single GAF domain that they contain can bind bilin(s) autocatalytically via heterologous recombination and then fluoresce, with potential applications as biomarkers and biosensors. Here, we report that a novel red/green CBCR GAF domain, SPI1085g2 from Spirulina subsalsa, covalently binds both phycocyanobilin (PCB) and phycoerythrobilin (PEB). The PCB-binding GAF domain exhibited canonical red/green photoconversion with weak fluorescence emission. However, the PEB-binding GAF domain, SPI1085g2-PEB, exhibited an intense orange fluorescence (λabs. max = 520 nm, λfluor. max = 555 nm), with a fluorescence quantum yield close to 1.0. The fluorescence of SPI1085g2-PEB was selectively and instantaneously quenched by copper ions in a concentration-dependent manner and exhibited reversibility upon treatment with the metal chelator EDTA. This study identified a novel PEB-binding cyanobacteriochrome-based fluorescent protein with the highest quantum yield reported to date and suggests its potential as a biosensor for the rapid detection of copper ions.
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참고문헌 (37)
참고문헌 신청
Ikeuchi M / 2008 / Cyanobacteriochromes : a new superfamily of tetrapyrrole-binding photoreceptors in cyanobacteria / Photochem. Photobiol. Sci 7:1159~1167
Rockwell NC / 2011 / Diverse two-cysteine photocycles in phytochromes and cyanobacteriochromes / Proc. Natl. Acad. Sci. USA 108:11854~11859
Rockwell NC / 2012 / Phycoviolobilin formation and spectral tuning in the DXCF cyanobacteriochrome subfamily / Biochemistry 51:1449~1463
Enomoto G / 2012 / Thiol-based photocycle of the blue and teal light-sensing cyanobacteriochrome Tlr1999 / Biochemistry 51:3050~3058
Rockwell NC / 2012 / Red/green cyanobacteriochromes : sensors of color and power / Biochemistry 51:9667~9677
Rockwell NC / 2011 / Diverse two-cysteine photocycles in phytochromes and cyanobacteriochromes / Proc. Natl. Acad. Sci. USA 108:11854~11859
Rockwell NC / 2012 / Phycoviolobilin formation and spectral tuning in the DXCF cyanobacteriochrome subfamily / Biochemistry 51:1449~1463
Enomoto G / 2012 / Thiol-based photocycle of the blue and teal light-sensing cyanobacteriochrome Tlr1999 / Biochemistry 51:3050~3058
Rockwell NC / 2012 / Red/green cyanobacteriochromes : sensors of color and power / Biochemistry 51:9667~9677
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