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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제3호
- 발행연도
- 2016.1
- 수록면
- 530 - 539 (10page)
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Bacterial light-oxygen-voltage-sensing photoreceptor-derived flavin mononucleotide (FMN)- based fluorescent proteins act as a promising distinct class of fluorescent proteins utilized for various biomedical and biotechnological applications. The key property of its independency towards oxygen for its chromophore maturation has greatly helped this protein to outperform the other fluorescent proteins such as GFP and DsRed for anaerobic applications. Here, we describe the feasibility of FMN-containing fluorescent protein FbFP as a metal-sensing probe by measuring the fluorescence emission changes of a protein with respect to the concentration of metal ions. In the present study, we demonstrated the mercury-sensing ability of FbFP protein and the possible amino acids responsible for metal binding. A ratiometric approach was employed here in order to exploit the fluorescence changes observed at two different emission maxima with respect to Hg2+ at micromolar concentration. The engineered variant FbFPC56I showed high sensitivity towards Hg2+ and followed a good linear relationship from 0.1 to 3 μM of Hg2+. Thus, further engineering with a rational approach would enable the FbFP to be developed as a novel and highly selective and sensitive biosensor for other toxic heavy metal ions as well.
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참고문헌 (26)
참고문헌 신청
Ayyadurai N / 2011 / Development of a selective, sensitive, and reversible biosensor by the genetic incorporation of a metal-binding site into green fluorescent protein / Angew. Chem. Int. Ed. Engl 50:6534~6537
Balint EE / 2013 / Fluorescence of a histidine-modified enhanced green fluorescent protein (EGFP) effectively quenched by copper (II) ions / J. Fluoresc 23:273~281
Buckley AM / 2015 / LOV-based reporters for fluorescence imaging / Curr. Opin. Chem. Biol 27:39~45
Chapleau RR / 2008 / Design o f a h ighly specific a nd n oninvasive b iosensor suitable for real-time in vivo imaging of mercury (II)uptake / Protein Sci 17:614~622
Davis SJ / 1998 / Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants / Plant Mol. Biol 36:521~528
Balint EE / 2013 / Fluorescence of a histidine-modified enhanced green fluorescent protein (EGFP) effectively quenched by copper (II) ions / J. Fluoresc 23:273~281
Buckley AM / 2015 / LOV-based reporters for fluorescence imaging / Curr. Opin. Chem. Biol 27:39~45
Chapleau RR / 2008 / Design o f a h ighly specific a nd n oninvasive b iosensor suitable for real-time in vivo imaging of mercury (II)uptake / Protein Sci 17:614~622
Davis SJ / 1998 / Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants / Plant Mol. Biol 36:521~528
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