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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제10호
- 발행연도
- 2020.1
- 수록면
- 1,552 - 1,558 (7page)
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초록· 키워드
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With an increase in the consumption of non-heated fresh food, foodborne shiga toxin-producing Escherichia coli (STEC) has emerged as one of the most problematic pathogens worldwide. Endolysin, a bacteriophage-derived lysis protein, is able to lyse the target bacteria without any special resistance, and thus has been garnering interest as a powerful antimicrobial agent. In this study, rV5-like phage endolysin targeting E. coli O157:H7, named as LysECP26, was identified and purified. This endolysin had a lysozyme-like catalytic domain, but differed markedly from the sequence of lambda phage endolysin. LysECP26 exhibited strong activity with a broad lytic spectrum against various gram-negative strains (29/29) and was relatively stable at a broad temperature range (4°C? 55°C). The optimum temperature and pH ranges of LysECP26 were identified at 37°C?42°C and pH 7? 8, respectively. NaCl supplementation did not affect the lytic activity. Although LysECP26 was limited in that it could not pass the outer membrane, E. coli O157: H7 could be effectively controlled by adding ethylenediaminetetraacetic acid (EDTA) and citric acid (1.44 and 1.14 log CFU/ml) within 30 min. Therefore, LysECP26 may serve as an effective biocontrol agent for gram-negative pathogens, including E. coli O157:H7.
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참고문헌 (37)
참고문헌 신청
Karen A. Krogfelt / 2004 / The Life of Commensal Escherichia coli in the Mammalian Intestine / EcoSal Plus 1(1)
Hunt JM / 2010 / Shiga toxin-producing Escherichia coli(STEC) / Clin. Lab. Med 30:21~45
Yang SC / 2017 / Current pathogenic Escherichia coli foodborne outbreak cases and therapy development / Arch. Microbiol 199:811~825
Pirnay JP / 2018 / The magistral phage / Viruses 10:64
Lin DM / 2017 / Phage therapy : an alternative to antibiotics in the age of multi-drug resistance / World J. Gastrointest. Pharmacol. Ther 8:162~173
Hunt JM / 2010 / Shiga toxin-producing Escherichia coli(STEC) / Clin. Lab. Med 30:21~45
Yang SC / 2017 / Current pathogenic Escherichia coli foodborne outbreak cases and therapy development / Arch. Microbiol 199:811~825
Pirnay JP / 2018 / The magistral phage / Viruses 10:64
Lin DM / 2017 / Phage therapy : an alternative to antibiotics in the age of multi-drug resistance / World J. Gastrointest. Pharmacol. Ther 8:162~173
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