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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 574 - 581 (8page)
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Next generation sequencing is commonly used to characterize the microbiome structure. MiSeq is commonly used to analyze the microbiome due to its relatively long read length. However, recently, Illumina introduced the 250x2 chip for HiSeq 2500. The purpose of this study was to compare the performance of MiSeq and HiSeq in the context of oral microbiome samples. The MiSeq Reagent Kit V3 and the HiSeq Rapid SBS Kit V2 were used for MiSeq and HiSeq 2500 analyses, respectively. Total read count, read quality score, relative bacterial abundance, community diversity, and relative abundance correlation were analyzed. HiSeq produced significantly more read sequences and assigned taxa compared to MiSeq. Conversely, community diversity was similar in the context of MiSeq and HiSeq. However, depending on the relative abundance, the correlation between the two platforms differed. The correlation between HiSeq and MiSeq sequencing data for highly abundant taxa (> 2%), low abundant taxa (2?0.2%), and rare taxa (0.2% >) was 0.994, 0.860, and 0.416, respectively. Therefore, HiSeq 2500 may also be compatible for microbiome studies. Importantly, the HiSeq platform may allow a high-resolution massive parallel sequencing for the detection of rare taxa.
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참고문헌 (25)
참고문헌 신청
Turnbaugh PJ / 2007 / The human microbiome project / Nature 449:804~810
Jorth P / 2014 / Metatranscriptomics of the human oral microbiome during health and disease / mBio 5:e01012~01014
Dong TS / 2019 / Influence of early life, diet, and the environment on the microbiome / Clin. Gastroenterol. Hepatol 17:231~242
Somineni HK / 2019 / The microbiome in patients with inflammatory diseases / Clin. Gastroenterol. Hepatol 17:243~255
Zackular JP / 2013 / The gut microbiome modulates colon tumorigenesis / mBio 4:e00692~00613
Jorth P / 2014 / Metatranscriptomics of the human oral microbiome during health and disease / mBio 5:e01012~01014
Dong TS / 2019 / Influence of early life, diet, and the environment on the microbiome / Clin. Gastroenterol. Hepatol 17:231~242
Somineni HK / 2019 / The microbiome in patients with inflammatory diseases / Clin. Gastroenterol. Hepatol 17:243~255
Zackular JP / 2013 / The gut microbiome modulates colon tumorigenesis / mBio 4:e00692~00613
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