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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.7
- 수록면
- 163 - 163 (1page)
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Oocyte vitrification utilizes an ultra-rapid freezing procedure using cryoprotectants and is widely used in assisted reproductive technologies for fertility preservation in women. The age-related quality decline in oocytes further deteriorates the vulnerable conditions of vitrification. Therefore, oocytes from older women need to be more attentive. Melatonin is an antioxidant that regulates many important physiological reactions, which contribute to the improvement of reproductive function. Thus, we aimed to investigate the effect of melatonin supplementation on the developmental potential of oocytes from aged mice after vitrification. Aged female mice (C57BL/6, 10-12 months old) were superovulated, and then MII oocytes were collected and randomly divided into control (AV) and melatonin (MAV) groups. Oocytes were subjected first to vitrification/warming and then fertilized in vitro and cultured up to the blastocyst to evaluate their developmental potentials. Melatonin (10-5 mol/L) was added to the vitrification/warming solution and culture medium during the experiment. The fresh oocytes (AF) without vi ... 전체 초록 보기
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