지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 75 - 85 (11page)
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초록· 키워드
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Background: Invasive infections due to foodborne pathogens, including Salmonella enterica serovar Typhimurium, are prevalent and life-threatening. This study aimed to evaluate the effects of ginsenoside Rg3 (Rg3) on the adhesion, invasion, and intracellular survival of S. Typhimurium.
Methods: The impacts of Rg3 on bacterial growth and host cell viability were determined using the time kill and the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assays, respectively. Gentamicin assay and confocal microscopic examination were undertaken to determine the effects of Rg3 on the adhesive and invasive abilities of S. Typhimurium to Caco-2 and RAW264.7 cells. Quantitative reverse transcription polymerase chain reaction was performed to assess the expression of genes correlated with the adhesion, invasion, and virulence of S. Typhimurium.
Results: Subinhibitory concentrations of Rg3 significantly reduced (p ＜ 0.05) the adhesion, invasion, and intracellular survival of S. Typhimurium. Rg3 considerably reduced (p ＜ 0.05) the bacterial motility as well as the release of nitrite from infected macrophages in a concentration-dependent manner. The expression of genes related to the adhesion, invasion, quorum sensing, and virulence of S. Typhimurium including cheY, hilA, OmpD, PrgK, rsgE, SdiA, and SipB was significantly reduced after Rg3 treatment. Besides, the compound downregulated rac-1 and Cdc-42 that are essential for actin remodeling and membrane ruffling, thereby facilitating Salmonella entry into host cells. This report is the first to describe the effects of Rg3 on “trigger” entry mechanism and intracellular survival S. Typhimurium.
Conclusion: Rg3 could be considered as a supplement agent to prevent S. Typhimurium infection.
Methods: The impacts of Rg3 on bacterial growth and host cell viability were determined using the time kill and the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assays, respectively. Gentamicin assay and confocal microscopic examination were undertaken to determine the effects of Rg3 on the adhesive and invasive abilities of S. Typhimurium to Caco-2 and RAW264.7 cells. Quantitative reverse transcription polymerase chain reaction was performed to assess the expression of genes correlated with the adhesion, invasion, and virulence of S. Typhimurium.
Results: Subinhibitory concentrations of Rg3 significantly reduced (p ＜ 0.05) the adhesion, invasion, and intracellular survival of S. Typhimurium. Rg3 considerably reduced (p ＜ 0.05) the bacterial motility as well as the release of nitrite from infected macrophages in a concentration-dependent manner. The expression of genes related to the adhesion, invasion, quorum sensing, and virulence of S. Typhimurium including cheY, hilA, OmpD, PrgK, rsgE, SdiA, and SipB was significantly reduced after Rg3 treatment. Besides, the compound downregulated rac-1 and Cdc-42 that are essential for actin remodeling and membrane ruffling, thereby facilitating Salmonella entry into host cells. This report is the first to describe the effects of Rg3 on “trigger” entry mechanism and intracellular survival S. Typhimurium.
Conclusion: Rg3 could be considered as a supplement agent to prevent S. Typhimurium infection.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (30)
참고문헌 신청
Scallan E / 2011 / Foodborne illness acquired in the United States-Major pathogens / Emerg Infect Dis 17:7~15
Kraehenbuhl JP / 2000 / Epithelial M cells : differentiation and function / Annu Rev Cell Dev Biol 16:301~332
Kage H / 2008 / Coordinated regulation of expression of Salmonella pathogenicity island 1 and flagellar type III secretion systems by ATP-dependent ClpXP protease / J Bacteriol 190:2470~2478
Lawhon SD / 2011 / Role of spi-1 secreted effectors in acute bovine response to Salmonella enterica serovar typhimurium : a systems biology analysis approach / PLoS One 6:e26869
Rosselin M / 2011 / Heterogeneity of type III secretion system(T3SS)-1-independent entry mechanisms used by Salmonella enteritidis to invade different cell types / Microbiology 157:839~847
Kraehenbuhl JP / 2000 / Epithelial M cells : differentiation and function / Annu Rev Cell Dev Biol 16:301~332
Kage H / 2008 / Coordinated regulation of expression of Salmonella pathogenicity island 1 and flagellar type III secretion systems by ATP-dependent ClpXP protease / J Bacteriol 190:2470~2478
Lawhon SD / 2011 / Role of spi-1 secreted effectors in acute bovine response to Salmonella enterica serovar typhimurium : a systems biology analysis approach / PLoS One 6:e26869
Rosselin M / 2011 / Heterogeneity of type III secretion system(T3SS)-1-independent entry mechanisms used by Salmonella enteritidis to invade different cell types / Microbiology 157:839~847
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