지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.2
- 수록면
- 11 - 16 (6page)
- DOI
- 10.7783/KJMCS.2021.29.1.11
이용수
초록· 키워드
오류제보하기
Background: The three Schisandracae species, Schisandra chinensis, Schisandra repanda, and Kadsura japonica, which are used as raw materials for the production of herbal medicines, are readily confused. However, of these three species, only S. chinensis is formally registered as a medicinal herb in the Korean Pharmacopeia. In order to facilitate the clear identification of S. chinensis, S. repanda, and K. japonica, we developed insertion or deletion (InDel) markers by comparing the chloroplast sequences of the three species.
Methods and Results: Genomic DNA was extracted from the leaves of S. chinensis, S. repanda, and K. japonica, and next generation sequencing (NGS) analysis was performed using the Illumina HiSeq-2500 platform. Chloroplast sequences for the three species were obtained and compared to identify polymorphic loci. Primers were designed for those InDel loci showing polymorphism in all three species, and four InDel markers were accordingly developed and successfully used for species discrimination.
Conclusions: In this study, we developed four markers that can be used to discriminate S. chinensis, S. repanda, and K. japonica at the molecular level.
Methods and Results: Genomic DNA was extracted from the leaves of S. chinensis, S. repanda, and K. japonica, and next generation sequencing (NGS) analysis was performed using the Illumina HiSeq-2500 platform. Chloroplast sequences for the three species were obtained and compared to identify polymorphic loci. Primers were designed for those InDel loci showing polymorphism in all three species, and four InDel markers were accordingly developed and successfully used for species discrimination.
Conclusions: In this study, we developed four markers that can be used to discriminate S. chinensis, S. repanda, and K. japonica at the molecular level.
목차
ABSTRACT
서언
재료 및 방법
결과 및 고찰
REFERENCES
참고문헌 (30)
참고문헌 신청
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Angiosperm Phylogeny Group(APG) / 2016 / An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants : APG IV / Botanical Journal of the Linnean Society 181:1~20
Clegg MT / 1994 / Rates and patterns of chloroplast DNA evolution / Proceedings of the National Academy of Sciences 91:6795~6801
Henry Daniell / 2016 / Chloroplast genomes: diversity, evolution, and applications in genetic engineering / Genome Biology 17(1)
Gao J / 2003 / Ribosomal DNA ITS sequences analysis of the Chinese crude drug fructus schisandrae sphenantherae and fruits of Schisandra viridis / China Journal of Chinese Materia Medica 28:706~710
Angiosperm Phylogeny Group(APG) / 2016 / An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants : APG IV / Botanical Journal of the Linnean Society 181:1~20
Clegg MT / 1994 / Rates and patterns of chloroplast DNA evolution / Proceedings of the National Academy of Sciences 91:6795~6801
Henry Daniell / 2016 / Chloroplast genomes: diversity, evolution, and applications in genetic engineering / Genome Biology 17(1)
Gao J / 2003 / Ribosomal DNA ITS sequences analysis of the Chinese crude drug fructus schisandrae sphenantherae and fruits of Schisandra viridis / China Journal of Chinese Materia Medica 28:706~710
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