지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.12
- 수록면
- 337 - 341 (5page)
- DOI
- 10.7841/ksbbj.2020.35.4.337
이용수
초록· 키워드
오류제보하기
Thermotolerant yeast, Kluyveromyces marxianus was applied for the production of 3-hydroxypropionic acid (3-HP), which is mainly used as a raw material for biodegradable polymers. The MCR (malonyl-CoA reductase) gene originated from the Chloroflexus aurantiacus strain was introduced into the engineered K. marxianus 17555-JBP2 strain showing improved xylose utilization in the previous study to develop the engineered K. marxianus JBP2_MCR strain. At 40℃, K. marxianus JBP2_MCR strain produced 0.14±0.00 g/L of 3-HP using xylose as a sole carbon source. As a result of measuring the enzymatic activity of malonyl-CoA reductase, the engineered K. marxianus JBP2_MCR strain exhibited 4.71 times higher MCR enzymatic activity than the parent strain. When 1.28 g/L malonyl-CoA was added into media, 1.58±0.18 g/L of 3-HP was produced by K. marxianus JBP2_MCR strain for 120 h.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (16)
참고문헌 신청
Fonseca, G. G. / 2008 / The yeast Kluyveromyces marxianus and its biotechnological potential / Applied microbiology and biotechnology 79:339~354
Anderson, P. / 1986 / High-efficiency carbohydrate fermentation to ethanol at temperatures above 40 C by Kluyveromyces marxianus var. marxianus isolated from sugar mills / Applied and environmental microbiology 51:1314~1320
Faga, B. A. / 2010 / Ethanol production through simultaneous saccharification and fermentation of switchgrass using Saccharomyces cerevisiae D5A and thermotolerant Kluyveromyces marxianus IMB strains / Bioresource technology 101:2273~2279
Margaritis, A. / 1982 / Direct fermentation of Dxylose to ethanol by Kluyveromyces marxianus strains / Applied and environmental microbiology 44:1039~1041
Zhang, B. / 2011 / Identification of a xylose reductase gene in the xylose metabolic pathway of Kluyveromyces marxianus NBRC1777 / Journal of industrial microbiology & biotechnology 38:2001~2010
Anderson, P. / 1986 / High-efficiency carbohydrate fermentation to ethanol at temperatures above 40 C by Kluyveromyces marxianus var. marxianus isolated from sugar mills / Applied and environmental microbiology 51:1314~1320
Faga, B. A. / 2010 / Ethanol production through simultaneous saccharification and fermentation of switchgrass using Saccharomyces cerevisiae D5A and thermotolerant Kluyveromyces marxianus IMB strains / Bioresource technology 101:2273~2279
Margaritis, A. / 1982 / Direct fermentation of Dxylose to ethanol by Kluyveromyces marxianus strains / Applied and environmental microbiology 44:1039~1041
Zhang, B. / 2011 / Identification of a xylose reductase gene in the xylose metabolic pathway of Kluyveromyces marxianus NBRC1777 / Journal of industrial microbiology & biotechnology 38:2001~2010
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