지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.47 No.3
- 발행연도
- 2020.9
- 수록면
- 693 - 705 (13page)
이용수
초록· 키워드
오류제보하기
Grapes (Vitis spp. L.) are the third most produced fruit in the world. Crown gall disease caused by Agrobacterium vitis forms galls in the stems of the grapevines and reduces the vitality of the fruit trees, resulting in reduced yields. This pathogen has occurred in vineyards worldwide and caused serious economic losses. It is a soil-borne disease, so Agrobacterium vitis can survive for several years in vineyards and is difficult to control. Additionally, since there is no effective chemical control method, the most effective control method is the breeding of resistant varieties. To make the resistant variety, marker-assisted selection (MAS) enables fast breeding with low cost. In this study, we applied a genome-wide association study (GWAS), by combining phenotyping and genotyping-by-sequencing (GBS), for the development of a single nucleotide polymorphism (SNP) marker set related to crown gall disease using 350 grapevine varieties. As a result of the GBS based genotyping analysis, about 58,635 SNPs were obtained. In addition, the phenotypic analysis showed 35.2% resistance, 73% moderate susceptibility and 16.4% highly susceptibility. Moreover, after confirmation, two genes (VvARF4 and VvATL6-like) were shown to be related to crown gall disease based on the results of GWAS analysis, using the phenotypic data, and GBS. High-resolution melting analysis (HRMA) was performed using the Luna<SUP>®</SUP> Universal Probe with real-time PCR to distinguish the melting peaks of the resistant and susceptible varieties. Our data show that these SNP markers are expected to be helpful in evaluating resistance against grapevine crown gall disease and in breeding.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Conclusion
References
참고문헌 (40)
참고문헌 신청
Bazzi C / 1991 / Hot-water treatment of grape propagation material : Its effects on Agrobacterium and on vine growth / Vitis 30:177~187
Bernardo R / 1998 / A model for marker-assisted selection among single crosses with multiple genetic markers / Theoretical and Applied Genetics 97:473~478
Botstein D / 1980 / Construction of a genetic linkage map in man using restriction fragment length polymorphisms / American Journal of Human Genetics 32:314~331
Bouzroud S / 2018 / Auxin response factors (ARFs) are potential mediators of auxin action in tomato response to biotic and abiotic stress (Solanum lycopersicum) / PLoS ONE 13:e0193517
Bradbury PJ / 2007 / TASSEL : Software for association mapping of complex traits in diverse samples / Bioinformatics 23:2633~2635
Bernardo R / 1998 / A model for marker-assisted selection among single crosses with multiple genetic markers / Theoretical and Applied Genetics 97:473~478
Botstein D / 1980 / Construction of a genetic linkage map in man using restriction fragment length polymorphisms / American Journal of Human Genetics 32:314~331
Bouzroud S / 2018 / Auxin response factors (ARFs) are potential mediators of auxin action in tomato response to biotic and abiotic stress (Solanum lycopersicum) / PLoS ONE 13:e0193517
Bradbury PJ / 2007 / TASSEL : Software for association mapping of complex traits in diverse samples / Bioinformatics 23:2633~2635
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Breeding of Light Purple Standard Rose ‘Swing Blue’ with Crown Gall Resistance
한국원예학회 학술발표요지
2015 .05
-
Agrobacterium tumefaciens from rose crown galls of different regions of Korea
한국농약과학회 학술발표대회 논문집
2018 .10
-
Transcriptional profiles of Rhizobium vitis-inoculated and salicylic acid-treated ‘Tamnara’ grapevines based on microarray analysis
Journal of Plant Biotechnology
2016 .01
-
Analysis of disease-resistant markers with cross-combination for breeding of Korean native chicken
한국가금학회 정기총회 및 학술발표회
2015 .11
-
Molecular characterization of tall fescue germplasm using SNP markers: population structure, linkage disequilibrium, and association mapping of yield-related traits
Plant Biotechnology Reports
2023 .04
-
포도 특성에 대한 전장 연관성 연구(GWAS) : 신품종 보호를 위한 표현 형질 관련 변이 탐색
한국원예학회 학술발표요지
2023 .05
-
Association Mapping and Development of SNP Marker Using GBS-Based SNPs in Apples
한국원예학회 학술발표요지
2018 .10
-
Development of SNP Marker Sets to Predict Genomic Estimated Breeding Values for Fruit Traits in Tomato
한국원예학회 학술발표요지
2021 .05
-
Genotype analysis for crossbreeds and Korean native chickens with disease resistance markers
한국가금학회 정기총회 및 학술발표회
2016 .11
-
Difference of Gall Formation Rates and Parasitic Rates of Thecodiplosis japonensis (Diptera: Ceidomyiidae) Larvae in Pine Forests around Urban and Mountain Villages
Journal of forest and environmental science
2020 .01
-
Genome-wide SNP Discovery Using Genotyping-By-Sequencing for Variety Identification in Korean Apple
한국원예학회 학술발표요지
2022 .05
-
Developing PCR-Based SNP Markers for Distinguishing Korean Waxy Corn F1 Hybrids
Plant Breeding and Biotechnology
2016 .01
-
Genome-wide SNP Identification in Two Hot Pepper Cultivars with Contrasting Bacterial Wilt Resistance Traits
한국원예학회 학술발표요지
2021 .05
-
Genome-wide SNP Discovery and Core Marker Set for DNA Barcoding in a Collection of Cultivated Peach
한국원예학회 학술발표요지
2022 .05
-
Development of SNP Marker Sets for Background Selection in Cultivated Tomato
한국원예학회 학술발표요지
2022 .11
-
Discrimination of chicken breed using high-density SNP markers
한국가금학회 정기총회 및 학술발표회
2017 .11
-
Single nucleotide polymorphism marker combinations for classifying Yeonsan Ogye chicken using a machine learning approach
한국축산학회지
2022 .09
-
수박 MAB용 마커세트 개발을 위한 핵심자원 대량 SNP 선발
한국원예학회 학술발표요지
2019 .10
-
Development of SNP marker set for markerassisted backcrossing (MABC) in cultivating tomato varieties
Korean Journal of Agricultural Science
2018 .09
-
Properties of Genotyping-by-sequencing Analysis and Validation the Accuracy of Selected SNP Markers
한국원예학회 학술발표요지
2017 .05
이 논문의 저자 정보
최근 본 자료
댓글(0)
0