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학술저널
저자정보
Lee, Yong-Sun (Department of Biochemistry and Molecular Genetics, University of Virginia, Institute of Molecular Biology and Genetics, School of Biological Sciences, Seoul National University) Jeon, Yesu (Department of Biochemistry and Molecular Genetics, University of Virginia, Institute of Molecular Biology and Genetics, School of Biological Sciences, Seoul National University) Park, Jong-Hoon (Department of Biochemistry and Molecular Genetics, University of Virginia, Institute of Molecular Biology and Genetics, School of Biological Sciences, Seoul National University) Hwang, Deog-Su (Institute of Molecular Biology and Genetics, School of Biological Sciences, Seoul National University) Dutta, Anindya (Department of Biochemistry and Molecular Genetics, University of Virginia)
저널정보
한국통합생물학회 Korean journal of biological sciences Korean journal of biological sciences 제8권 제3호
발행연도
2004.1
수록면
213 - 220 (8page)

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MicroRNA and siRNA (small interfering RNA), representative members of small RNA, exert their effects on target gene expression through association with protein complexes called miRNP (microRNA associated ribonucleoproteins) and RISC (RNA induced silencing complex), respectively. Although the protein complexes are yet to be fully characterized, human EIF2C2 protein has been identified as a component of both miRNP and RISC. In this report, we raised antiserum against EIF2C2 in order to begin understanding the protein complexes. An immunoblot result indicates that EIF2C2 protein is ubiquitously expressed in a variety of cell lines from human and mouse. EIF2C2 protein exists in both cellular compartments, as indicated by an immunoblot assay with a nuclear extract and a cytosolic fraction (S100 fraction) from HeLa S3 lysate. Depletion of EIF2C1 or EIF2C2 protein resulted in a decrease of microRNA, suggesting a possible role of these proteins in microRNA stability or biogenesis. We also prepared antiserum against dsRNA binding protein PACT, whose homologs in C. elegans and Drosophila are known to have a role in the RNAi (RNA interference) pathway. The expression of PACT protein was also observed in a wide range of cell lines.

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