Dominance Relationship between Two Self-Incompatible Brassica campestris Haplotypes in Response to $CO_2$ Gas

Hyun, Ji-Young; Gothandam, Kodiveri Muthukalianan; Baek, Nam-Kwon; Wang, Gongwei; Chung, Yong-Yoon

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자료유형: 학술저널

저자정보: Hyun, Ji-Young (School of Life Sciences and Biotechnology, Korea University) Gothandam, Kodiveri Muthukalianan (School of Life Sciences and Biotechnology, Korea University) Baek, Nam-Kwon (School of Life Sciences and Biotechnology, Korea University) Wang, Gongwei (School of Life Sciences and Biotechnology, Korea University) Chung, Yong-Yoon (School of Life Sciences and Biotechnology, Korea University)

저널정보: 한국식물학회 식물학회지 식물학회지 제50권 제2호

발행연도: 2007.1

수록면: 161 - 166 (6page)

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In Brassica, self-incompatibility (SI) can be overcome by $CO_2$ application, an effective method for obtaining numerous inbred lines for $F_1$ commercial seed. We previously reported two different S-alleles of Brassica campestris, $S^{733}\;and\;S^{734}$, with extremely different degrees of susceptibility to this gas. In the current study, we raised a cross-population between those two genetic lines, and analyzed their reaction level of self-incompatibility to $CO_2(RLSICO_2)$. Here, all 40 of our progeny from the $F_1$ cross-population were susceptible, maintaining high values of $RLSICO_2$. This suggests that the susceptible line, $S^{734}$, is dominant to the insusceptible line, $S^{733}$. We also generated an $F_2$ selfing-population of each crossed progeny, $S^{733}female\;S^{734}male\;and\;S^{733}maleS^{734}female$, to assess the $RLSICO_2$ of each individual. PCR-RFLP analysis was performed to determine the S-genotype of the $F_2$ population. The $S^{734}$ allele segregated in a theoretical ratio of the dominant trait, and the $RLSICO_2$ was consistent with the dominance relationship. Therefore, we have now demonstrated that high $RLSICO_2$ in B. campestris is controlled by a dominant gene.

#dominant #PCR-RFLP #reaction level of self-incompatibility #S locus receptor kinase

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