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자료유형
학술저널
저자정보
Kormutak, A. (Institute of Plant Genetics and Biotechnology Slovak Academy of Sciences) Hong, Y.-P. (Korea Forest Research Institute, Department of Forest Genetic Resources) Kwon, H.-Y. (Korea Forest Research Institute, Department of Forest Genetic Resources) Kim, C.-S. (Warm-temperature Forest Research Center, Korea Forestry Research Institute)
저널정보
한국산림과학회(구 한국임학회) 한국산림과학회지 한국산림과학회지 제96권 제2호
발행연도
2007.1
수록면
131 - 137 (7page)

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The first evidence has been provided about the variation within trnL-trnV and trnF-trnT spacer regions of cpDNAs in Korean fir and Manchurian fir, revealed by PCR-RFLP analysis. Four cpDNA haplotypes have accordingly been recognized by being analyzed using the trnL-trnV/Tru11 primer-enzyme combination and 3 haplotypes using the trnF-trnT/TagI combination, which exhibited inter and intraspecific variation. A total of 6 cpDNA haplotypes were recognized by pooling the PCR-RFLP variants observed in both combinations. Haplotypes 2 and 3 were common for both species investigated, whereas haplotypes 1, 4, and 5 were detected only in Korean fir and haplotype 6 was detected only in Manchurian fir. Although haplotypes 2 and 3 were common in both species, haplotype 2 was major haplotype for Korean fir and haplotype 3 was one of the 2 major haplotypes for Manchurian fir. Restricted occurrence of haplotype 4 in Mt. Halla and haplotype 5 in Mt. Jiri of the Korean fir may represent the existence of geographic isolation by the sea between them. Diagnostic potential of individual haplotypes in discriminating between the two species as well as between their populations is discussed.

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