지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2004.1
- 수록면
- 235 - 240 (6page)
이용수
초록· 키워드
오류제보하기
In vitro maturation of denuded porcine immature oocytes can be enhanced by co-incubation with spermatozoa even before fertilization. This study was to determine whether the addition of spermatozoa into the culture medium could influence the nuclear maturation of porcine cumulus-enclosed germinal vesicle (GV) oocytes. Cumulus-oocyte complexes (COCs) were collected from follicles of 3- to 5-mm diameter. Porcine COCs were cultured in tissue culture medium containing spermatozoa. After 48 h culture, oocytes were examined for evidence of GV breakdown, metaphase I, anaphase-telophase I, and metaphase II. The proportion of oocytes reaching at metaphase II was significantly (P < 0.05) increased in the oocytes cultured in media containing spermatozoa compared to those in media without spermatozoa (52.3% vs 12.5%). No difference in the percentage of metaphase II was observed among the different periods of spermatozoa exposure and among the spermatozoa from different species. The proportion of oocytes reaching metaphase II was significantly different between high and low concentrations of spermatozoa. The present study suggests that manunalian spermatozoa contain a substance(s) that improves nuclear in vitro maturation of porcine cumulus-enclosed GV oocytes. Enhancing effect of spermatozoa for in vitro maturation of oocytes is a highly dose-dependent.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Effect of Co-culture with Spermatozoa on the Resumption of Meiosis in Porcine Germinal Vesicle Oocytes Arrested with Meiotic Inhibitors
한국동물번식학회지
2005 .01
-
Effect of Mammalian Spermatozoa on In Vitro Maturation of Porcine Germinal Vesicle Oocyte in Chemically Defined Medium
한국동물번식학회 학술대회
2002 .01
-
돼지난자의 체외성숙에 영향을 미치는 요인
한국수정란이식학회지
1996 .01
-
Stimulatory Effect of Porcine Epididymal Fluid on In Vitro Maturation of Porcine Immature Oocytes
한국동물번식학회지
2006 .01
-
Effect of Porcine Epididymal Fluid on In Vitro Maturation of Porcine Germinal Vesicle Oocyte
한국동물번식학회지
2008 .01
-
돼지난포의 배양에 의한 난자의 성숙
한국동물번식학회지
1997 .01
-
도축장에서의 난소운반 온도가 돼지난포란의 체외성숙에 미치는 영향
한국동물번식학회지
1998 .01
-
Relationship between In Vitro Maturation and Plasminogen Activator Activity on Porcine Cumulus-Oocytes Complexes Exposed to Oxidative Stress
한국동물번식학회지
2011 .01
-
배란직전 생쥐 난포란의 체외성숙, 수정 및 배 발달에 미치는 전배양의 교과에 관한 연구
한국동물번식학회지
1990 .01
-
난자성숙시간에 따른 처녀발생유기 돼지난자의 초기발생
한국수정란이식학회지
1994 .01
-
Effects of Genotypes on In Vitro Maturation and Fertilization of Frozen-Thawed Porcine Oocytes
한국동물번식학회지
2005 .01
-
Capacitation and acrosome reaction differences of bovine, mouse and porcine spermatozoa in responsiveness to estrogenic compounds
한국축산학회지
2014 .01
-
Fertilization Processes in Porcine Oocytes Following Intracytoplasmic Injection of Porcine and Mouse Spermatozoa
한국동물번식학회 학술대회
2001 .01
-
돼지 미성숙 난포란의 유리화 동결융해후 FDA 처리가 체외수정과 배 발육에 미치는 영향 II. 난구세포의 부착정도가 돼지난포란의 체외성숙에 미치는 영향
한국수정란이식학회지
1996 .01
-
In Vitro Maturation of Porcine Oocytes in a Dry Incubator without $CO_2$ Gas Supplement
한국동물번식학회지
2012 .01
-
Establishment of Normal Reference Data of Analysis in the Fresh and Cryopreserved Canine Spermatozoa
한국수정란이식학회지
2018 .01
-
Effects of Ovary Status and In Vitro Maturation Condition on the Developmental Competence of Canine Oocytes
한국수정란이식학회지
2012 .01
-
소 난자의 체외성숙시 난구세포와 난세포질에서 다르게 발현되는 Matrix Metalloproteinases의 분석
한국수정란이식학회지
2018 .01
-
Effect of Epididymal Fluid Fractionated by Chromatography on In Vitro Maturation of Porcine Follicular Oocytes
한국동물번식학회지
2010 .01
-
Porcine Oocytes with Meiotic Competence are Synchronized Early Stage of Germinal Vesicle (GV)
한국동물번식학회지
2010 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0