지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 1 - 7 (7page)
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Background Endocrine disruptors are exogenous substance, interfere with the endocrine system, and disrupt hormonal functions. However, the effect of endocrine disruptors in different species has not yet been elucidated. Therefore, we investigated the possible effects of 17ss-estradiol (E2), progesterone (P4), genistein (GEN) and 4-tert-octylphenol (OP), on capacitation and the acrosome reaction in bovine, mouse, and porcine spermatozoa. In this in vitro trial, spermatozoa were incubated with 0.001-100 muM of each chemical either 15 or 30 min and then assessed capacitation status using chlortetracycline staining.
Results E2 significantly increased capacitation and the acrosome reaction after 30 min, while the acrosome reaction after 15 min incubation in mouse spermatozoa. Simultaneously, capacitation and the acrosome reaction were induced after 15 and 30 min incubation in porcine spermatozoa, respectively. Capacitation was increased in porcine spermatozoa after 15 min incubation at the lowest concentration, while the acrosome reaction was increased in mouse spermatozoa after 30 min (P <0.05). E2 significantly increased the acrosome reaction in porcine spermatozoa, but only at the highest concentration examined (P <0.05). P4 significantly increased the acrosome reaction in bovine and mouse spermatozoa treated for 15 min (P <0.05). The same treatment significantly increased capacitation in porcine spermatozoa (P <0.05). P4 significantly increased capacitation in mouse spermatozoa treated for 30 min (P <0.05). GEN significantly increased the acrosome reaction in porcine spermatozoa treated for 15 and 30 min and in mouse spermatozoa treated for 30 min (P <0.05). OP significantly increased the acrosome reaction in mouse spermatozoa after 15 min (P <0.05). Besides, when spermatozoa were incubated for 30 min, capacitation and the acrosome reaction were higher than 15 min incubation in E2 or GEN. Furthermore, the responsiveness of bovine, mouse and porcine spermatozoa to each chemical differed.
Conclusions In conclusion, all chemicals studied effectively increased capacitation and the acrosome reaction in bovine, mouse, and porcine spermatozoa. Also we found that both E2 and P4 were more potent than environmental estrogens in altering sperm function. Porcine and mouse spermatozoa were more responsive than bovine spermatozoa.
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참고문헌 (24)
참고문헌 신청
Adeoya-Osiguwa SA / 2003 / 17beta- Estradiol and environmental estrogens significantly affect mammalian sperm function / Hum Reprod 18 : 100 ~ 107
Akingbemi BT / 2001 / Oestrogenic and antiandrogenic chemicals in the environment: effects on male reproductive health / Ann Med 33 : 391 ~ 403
Couse JF / 1999 / Estrogen receptor null mice: what have we learned and where will they lead us? / Endocr Rev 20 : 358 ~ 417
Durkee TJ / 1998 / Identification of estrogen receptor protein and messenger ribonucleic acid in human spermatozoa / Am J Obstet Gynecol 178 : 1288 ~ 1297
Fraser LR / 2006 / Effects of estrogenic xenobiotics on human and mouse spermatozoa / Hum Reprod 21 : 1184 ~ 1193
Akingbemi BT / 2001 / Oestrogenic and antiandrogenic chemicals in the environment: effects on male reproductive health / Ann Med 33 : 391 ~ 403
Couse JF / 1999 / Estrogen receptor null mice: what have we learned and where will they lead us? / Endocr Rev 20 : 358 ~ 417
Durkee TJ / 1998 / Identification of estrogen receptor protein and messenger ribonucleic acid in human spermatozoa / Am J Obstet Gynecol 178 : 1288 ~ 1297
Fraser LR / 2006 / Effects of estrogenic xenobiotics on human and mouse spermatozoa / Hum Reprod 21 : 1184 ~ 1193
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