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논문 기본 정보

자료유형
학술저널
저자정보
Park S. B. (Department of Animal Science, Daegu University) Park K S. (Department of Obstetrics and Gynecology, Kyungpook National University Hospital) Lee T. H. (Department of Obstetrics and Gynecology, Kyungpook National University Hospital) Chun S. S. (Department of Obstetrics and Gynecology, Kyungpook National University Hospital) Kim K S. (Cheonan Yonam College) Song H. B. (Department of Animal Science, Daegu University)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제28권 제4호
발행연도
2004.1
수록면
227 - 233 (7page)

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This study was conducted to investigate the effect of various addition and exclusion time of glucose (Control: no addition, A: 24~72 h, B: 24~48 h, C: 48~72 h, D: 0~72 h, E: 0~48 h, F: 0~24 h and 48~72 h, G: 0~24 h) on embryonic developmental capacity of 2-cell embryos in mice. Developed blastocysts were assessed for mean cell number by differential staining. The zona-intact blastocyst (ZiB) rates were higher (p<0.05) in group B than control. However, the zona-escape blastocyst (ZeB) rates were not significantly different in all groups. At 72 h, total blastocyst (ZiB + ZeB) formation rates were not significantly different in all groups. The mean cell number was not significantly different among all groups. The inner cell mass (ICM) cell number was higher (p<0.05) in group F than control, group A, B and G. The trophectoderm (TE) cell number was higher (p<0.05) in control than group A and D. The %ICM was higher (p<0.05) in group C, D and F than control. The ICM : TE ratio was not significantly different in all groups. Between control and glucose group, no significant difference was observed in the total blastocysts (ZiB + ZeB) formation rates. Also, no significant difference was observed in the mean cell number, ICM cell number and ICM : TE ratio. However the TE cell number was higher (p<0.05) in control than glucose group and %ICM was higher (p<0.05) in glucose group than control. In conclusion, glucose added in culture medium was not inhibitory on blastocyst formation but glucose added for 48 ~72 h in culture medium increases %ICM of blastocysts in mice.

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