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논문 기본 정보

자료유형
학술대회자료
저자정보
Park, Sung-Baek (Division of Life Resources, Daegu University) Park, Kee-Sang (Division of Life Resources, Daegu University, Department of OB/Gyn, Kyungpook National University Hospital) Lee, Taek-Hoo (Department of OB/Gyn, Kyungpook National University Hospital) Chun, Sag-Sik (Department of OB/Gyn, Kyungpook National University Hospital) Song, Hai-Bum (Division of Life Resources, Daegu University)
저널정보
한국동물번식학회 한국동물번식학회 학술대회 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
발행연도
2003.1
수록면
66 - 66 (1page)

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초록· 키워드

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The aim of this study was to investigate effect of different energy substrates on embryonic development of mouse embryos. Two cell embryos, recovered from ICR female mice (4 weeks old) at 44~52hrs after hCG injection (mated just after hCG injection), were cultured fur 72 hrs in the medium (MEM) supplemented with the three different energy substrates [glucose(G), pyruvate(P) and lactate(L)] and combinations (Control: 0 mM: group A: G 0.5; B: G 3.15; C: P 0.1; D: P 0.32; E: L 5.87; F: L 10.5; G: G0.5+P0.32+L10.5; H: G3.15+P0.1+L5.87; I: G0.5+P0.1+L5.87; J: G3.15+P0.32+L10.5). Blastocysts were stained differentially using PI and bisbenzimide. The 69.8% of the 2 cell embryos cultured in group F were developed the blastocysts. This was the highest (NS) than all other tested groups (44.2~62.8%). Blastocysts, cultured in the group E (60.4$\pm$26.9) and G (58.1$\pm$26.3), had significantly(p<0.05: group E vs. control, B, C, D; G vs. control, A, B, C, D) higher mean cell number compared with the other (42.6$\pm$25.8 ~ 55.2$\pm$31.3) and control (42.6$\pm$25.8) was at the basal level. The proportion of ICM (% ICM of total cells) in blastocysts cultured in group B (26.0$\pm$9.5%), C (29.6$\pm$22.8%) and J (26.0$\pm$11.8%) were significantly higher (p<0.05: control vs. group B, C, J: A vs. C, J; C vs. D, E, I) than those of other tested groups (15.0$\pm$10.6 ~ 23.8$\pm$ 12.9%) and control (15.0$\pm$10.6%) was at the basal level. These results showed that energy substrates supported the development of mouse 2 cell embryos, especially with greater embryo development in high dose of lactate added to media.

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