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자료유형
학술저널
저자정보
송대빈 (Division of Agricultural Engineering, Gyeongsang National University/Institute of Agricultural & Life Science) 정효석 (Division of Agricultural Engineering, Gyeongsang National University/Institute of Agricultural & Life Science) 김성태 (Division of Agricultural Engineering, Gyeongsang National University/Institute of Agricultural & Life Science)
저널정보
한국농업기계학회 바이오시스템공학(구 한국농업기계학회지) 바이오시스템공학 제32권 제6호
발행연도
2007.1
수록면
455 - 461 (7page)

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The electric current of a flow injection type enzyme-sensor was measured to confirm the stable operating conditions of the sensor. The current of the sensor was decreased as the buffer solution velocity increased. Under the limitation of the cycle time to be below 10 minutes, the effective ranges of the buffer solution velocity were suggested $0.10{\sim}0.26$, $0.12{\sim}0.24$, $0.1{\sim}0.25$ and $0.05{\sim}0.10\;cm/s$ of 1.0, 1.4, 2.4 and 3.4 mm of the electrode diameters, respectively. As the reaction time of the enzyme and the substrate was increased, the current was decreased because of the dilution between the sample and buffer solution. Therefore, it could be recommended that the reaction time was able to be selected as shortly as possible in consideration of the total cycle time. As the result of the experiments using a different volume ratio of the enzyme to substrate, it was concluded that the substrate had to be mixed with the same amount of the enzyme. The current have increased remarkably in proportion to the electrode diameter under 0.1 cm/s of the buffer solution velocity but there was no difference over 0.1 cm/s of the buffer solution velocity. The cross type arrangement of the electrode was highly suggested for application and machining of the sensor.

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