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학술저널
저자정보
Yoon, Ji-Hwan (Department of Microbiology, Dankook University) Park, Ji-Eun (Department of Microbiology, Dankook University) Suh, Dong-Yeon (Department of Microbiology, Dankook University) Hong, Seung-Beom (Korean Agricultural Culture Collection and National Institute of Agricultural Science and Technology, Rural Development Administration) Ko, Seung-Ju (Korean Agricultural Culture Collection and National Institute of Agricultural Science and Technology, Rural Development Administration) Kim, Seong-Hwan (Department of Microbiology, Dankook University)
저널정보
한국균학회 Mycobiology Mycobiology 제35권 제1호
발행연도
2007.1
수록면
21 - 24 (4page)

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To evaluate which dye is effective in a plate assay for detecting extracellular cellulase activity produced by fungi, four chromogenic dyes including remazol brilliant blue, phenol red, congo red, and tryphan blue, were compared using chromagepic media. For the comparison, 19 fungal species belonging to three phyla, ascomycota, basidiomycota, and zygomycota were inoculated onto yeast nitrogen-based media containing different carbon substrates such as cellulose (carboxylmethyl and avicel types) and cellobiose labeled with each of the four dyes. Overall, the formation of clear zone on agar media resulting from the degradation of the substrates by the enzymes secreted from the test fungi was most apparent with media containing congo red. The detection frequency of cellulase activity was also most high on congo red-supplemented media. The results of this study showed that congo red is better dye than other three dyes in, a plate assay for, fungal enzyme detection.

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