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논문 기본 정보

자료유형
학술저널
저자정보
Song, Nam-Yoong (Division of Optical Metrology, Korea Research Institute of Standards & Science) Kim, Hyong-Ha (Organic and Biological Analysis Group, Division of Chemical Metrology and Materials Evaluation, Korea Research Institute of Standards & Science) Park, Tae-Sook (Division of Optical Metrology, Korea Research Institute of Standards & Science, Molecular/Nano photochemistry & Photonics Laboratory, Department of Chemistry, Chungnam National) Yoon, Min-Joong (Molecular/Nano photochemistry & Photonics Laboratory, Department of Chemistry, Chungnam National University)
저널정보
한국광과학회 Journal of photoscience : an international journal officail organ of the korean society of photoscience Journal of photoscience : an international journal officail organ of the korean society of photoscience 제12권 제2호
발행연도
2005.1
수록면
87 - 93 (7page)

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A new assay technique to distinguish between pure compounds and the isomeric mixtures has been suggested using single molecule (SM) fluorescence detection technique. Since the number of emission spots in a fluorophorespread film prepared from a genuine dye solution was determined by experimental condition, the deviation of spot numbers from the expected values could be considered to be an indication of lower purity of the sample solution. The lower limit of sample concentration for this assay was determined to be $5{\times}10^{-10}$ M to show uniform number of expected spots within 10% uncertainties in our experimental condition. An individual fluorescence intensity distribution for a mixture of isomers having doubly different emissivities was simulated by adding distributions obtained from Cy3 and nile red (NR) independently. The result indicated that the mixture could be identified from the pure compounds through the difference in the number of Gaussian functions to fit the distribution. This new assay technique can be applied to the purity test for synthetic biofluorophores which are usually prepared in small quantities not enough for classical ensemble assays.

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