Assessment of Feasibility for Developing Toxicogenomics Biomarkers by comparing in vitro and in vivo Genomic Profiles Specific to Liver Toxicity Induced by Acetaminophen

Kang, Jin-Seok; Jeong, Youn-Kyoung; Suh, Soo-Kyung; Kim, Joo-Hwan; Lee, Woo-Sun; Lee, Eun-Mi; Shin, Ji-He; Jung, Hai-Kwan; Kim, Seung-Hee; Park, Sue-Nie

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자료유형: 학술저널

저자정보: Kang, Jin-Seok (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration) Jeong, Youn-Kyoung (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration) Suh, Soo-Kyung (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration) Kim, Joo-Hwan (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration) Lee, Woo-Sun (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration) Lee, Eun-Mi (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration) Shin, Ji-He (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration) Jung, Hai-Kwan (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration) Kim, Seung-Hee (Department of To) Park, Sue-Nie

저널정보: 대한독성유전단백체학회 Molecular & cellular toxicology Molecular & cellular toxicology 제3권 제3호

발행연도: 2007.1

수록면: 177 - 184 (8page)

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As a possible feasibility of the extrapolation between in vivo and in vitro systems, we investigated the global gene expression from both mouse liver and mouse hepatic cell line treated with hepatotoxic chemical, acetaminophen (APAP), and compared between in vivo and in vitro genomic profiles. For in vivo study, mice were orally treated with APAP and sacrificed at 6 and 24 h. For in vitro study, APAP were administered to a mouse hepatic cell line, BNL CL.2 and sampling was carried out at 6 and 24 h. Hepatotoxicity was assessed by analyzing hepatic enzymes and histopathological examination (in vivo) or lactate dehydrogenase (LDH) assay and morphological examination (in vitro). Global gene expression was assessed using microarray. In high dose APAPtreated group, there was centrilobular necrosis (in vivo) and cellular toxicity with the elevation of LDH (in vitro) at 24 h. Statistical analysis of global gene expression identified that there were similar numbers of altered genes found between in vivo and in vitro at each time points. Pathway analysis identified glutathione metabolism pathway as common pathways for hepatotoxicty caused by APAP. Our results suggest it may be feasible to develop toxicogenomics biomarkers or profiles by comparing in vivo and in vitro genomic profiles specific to this hepatotoxic chemical for application to prediction of liver toxicity.

#Acetaminophen #in vivo #in vitro #Toxicogenomics #Hepatotoxicity #Microarray

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이 논문의 저자 정보

Kang, Jin-Seok

소속기관 Department of Biomedical Laboratory Science, Namseoul University

주요연구분야 자연과학 > 생물학

논문수 7 이용수 1

Jeong, Youn-Kyoung

소속기관 Division of Genetic Toxicology, National Institute of Toxicological Research, Korea Food and Drug Ad

주요연구분야 자연과학 > 생물학 의약학 > 약학

논문수 5 이용수 0

Suh, Soo-Kyung

소속기관 Division of Genetic Toxicology, National Institute of Toxicological Research, Korea Food and Drug Ad

주요연구분야 자연과학 > 생물학 의약학 > 약학

논문수 7 이용수 0

Kim, Joo-Hwan