지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 717 - 723 (7page)
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초록· 키워드
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A rapid and specific PCR assay for the simultaneous detection of Listeria monocytogenes, Escherichia coli O157:H7, Bacillus cereus, Salmonella spp., and Staphylococcus aureus in foods was developed to reduce the detection time and to increase sensitivity. Multiplex PCR developed in this study produced only actA, fliC, hbl, invA, ileS amplicons, but did not produce any non-specific amplicon. The primer sets successfully amplified the target genes in the multiplex PCR without any non-specific or additional bands on the other strains. The multiplex PCR assays also amplified some target genes from five pathogens, and multiplex amplification was obtained from as little as 1 pg of DNA. According to the results from the sensitivity evaluation, the multiplex PCR developed in this study detected 10 cells/mL of the pathogens inoculated in milk samples, respectively. The results suggested that multiplex PCR was an effective assay demonstrating high specificity for the simultaneous detection of five target pathogens in food system.
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참고문헌 (21)
참고문헌 신청
Abd-Elmagid, A / 2013 / Discriminatory simplex and multiplex PCR for four species of the genus Sclerotinia / J. Microbiol. Meth 92:293~300
Amagliani, G / 2004 / Direct detection of Listeria monocytogenes from milk by magnetic based DNA isolation and PCR / Food Microbiol 21:597~603
Bhagwat, A. A / 2003 / Simultaneous detection of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella strains by real-time PCR / Int. J. Food Microbiol 84:217~224
Chiang, Y. C / 2012 / Multiplex PCR and a chromogenic DNA macroarray for the detection of Listeria monocytogens, Staphylococcus aureus, Streptococcus agalactiae, Enterobacter sakazakii, Escherichia coli O157:H7, Vibrio parahaemolyticus, Salmonella spp. and Pseudomonas fluorescens in milk and meat samples / J. Microbiol. Meth 88:110~116
Cremonesi, P / 2005 / Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products / Mol. Cell. Probe 19:299~305
Amagliani, G / 2004 / Direct detection of Listeria monocytogenes from milk by magnetic based DNA isolation and PCR / Food Microbiol 21:597~603
Bhagwat, A. A / 2003 / Simultaneous detection of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella strains by real-time PCR / Int. J. Food Microbiol 84:217~224
Chiang, Y. C / 2012 / Multiplex PCR and a chromogenic DNA macroarray for the detection of Listeria monocytogens, Staphylococcus aureus, Streptococcus agalactiae, Enterobacter sakazakii, Escherichia coli O157:H7, Vibrio parahaemolyticus, Salmonella spp. and Pseudomonas fluorescens in milk and meat samples / J. Microbiol. Meth 88:110~116
Cremonesi, P / 2005 / Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products / Mol. Cell. Probe 19:299~305
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