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자료유형
학술저널
저자정보
Lee, Won-Jae (College of Veterinary Medicine, Kyungpook National University) Park, Ji-Sung (College of Veterinary Medicine, Gyeongsang National University) Lee, HyeonJeong (College of Veterinary Medicine, Gyeongsang National University) Lee, Seung-Chan (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) Lee, Jeong-Hyun (College of Veterinary Medicine, Gyeongsang National University) Ock, Sun-A (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) Rho, Gyu-Jin (College of Veterinary Medicine, Gyeongsang National University) Lee, Sung-Lim (College of Veterinary Medicine, Gyeongsang National University)
저널정보
한국동물번식학회 한국수정란이식학회지 한국수정란이식학회지 제32권 제3호
발행연도
2017.1
수록면
209 - 220 (12page)

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The estrogen-mediated effect of mesenchymal stem cells (MSCs) is a highly critical factor for the clinical application of MSCs. However, the present study is conducted on MSCs derived from adult donors, which have different physiological status with steroid hormonal changes. Therefore, we explores the important role of $17{\beta}$-estradiol (E2) in MSCs derived from female and male newborn piglets (NF- and NM-pBMSCs), which are non-sexually matured donors with steroid hormones. The results revealed that in vitro treatment of MSCs with E2 improved cell proliferation, but the rates varied according to the gender of the newborn donors. Following in vitro treatment of newborn MSCs with E2, mRNA levels of Oct3/4 and Sox2 increased in both genders of MSCs and they may be correlated with both estrogen receptor ${\alpha}$ ($ER{\alpha}$) and $ER{\beta}$ in NF-pBMSCs, but NM-pBMSCs were only correlated with $ER{\alpha}$. Moreover, E2-treated NF-pBMSCs decreased in ${\beta}$-galactosidase activity but no influence on NM-pBMSCs. In E2-mediated differentiation capacity, E2 induced an increase in the osteogenic and chondrogenic abilities of both pBMSCs, but adipogenic ability may increased only in NF-pBMSCs. These results demonstrate that E2 could affect both genders of newborn donor-derived MSCs, but the regulatory role of E2 varies depending on gender-dependent characteristics even though the original newborn donors had not been affected by functional steroid hormones.

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