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논문 기본 정보

자료유형
학술저널
저자정보
Lee, Yong-Hee (Department of Biotechnology, College of Engineering, Daegu University) Kim, Jin-Woo (Department of Biotechnology, College of Engineering, Daegu University) Chae, Sung-Kyu (Department of Biotechnology, College of Engineering, Daegu University) Ahn, Jae-Hyun (Department of Biotechnology, College of Engineering, Daegu University) Do, Geon-Yeop (Department of Biotechnology, College of Engineering, Daegu University) Koo, Deog-Bon (Department of Biotechnology, College of Engineering, Daegu University)
저널정보
한국동물번식학회 한국수정란이식학회지 한국수정란이식학회지 제30권 제1호
발행연도
2015.1
수록면
23 - 31 (9page)

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Catalpol, an iridoid glucoside, isolated from the root of Rehmannia glutinosa Libosch. It possesses a broad range of biological and pharmacological activity including anti-tumor, anti-inflammation and anti-oxidant by acting as a free radical scavenger. Therefore, in this study, the effects of catalpol on blastocyst development, expression levels of reactive oxygen species (ROS) and apoptotic index were investigated in porcine embryos. After in vitro maturation and fertilization, porcine embryos were cultured for 6 days in porcine zygote medium 3 (PZM-3) supplemented with catalpol (0, 100, 200 and $400{\mu}M$, respectively). Blastocyst development not significantly improved in the catalpol treated group when compared with control group. Otherwise, the intracelluar levels of ROS were decreased and the numbers of apoptotic nuclei were reduced in the catalpol ($100{\mu}M$) treated porcine blastocysts (P<0.05). On the other hand, blastocyst development was significantly improved in the catalpol ($100{\mu}M$) treated group when compared with the untreated catalpol group under $H_2O_2$ ($200{\mu}M$) induced oxidative stress (P<0.05). Otherwise, the intracellular levels of ROS in catalpol ($100{\mu}M$) treated group were significantly decreased in the untreated catalpol group under $H_2O_2$ ($200{\mu}M$) induced oxidative stress (P<0.05). Furthermore, the total cell numbers of blastocysts were significantly increased (P<0.05) in the catalpol ($100{\mu}M$) treated group under $H_2O_2$ ($200{\mu}M$) induced oxidative stress, whereas numbers of apoptoic nuclei were significantly reduced (P<0.05). In conclusion, our results indicate that treatment of catalpol may have important implications for improving developmental competence and preimplantation quality of porcine embryos through its anti-oxidant and anti-apoptotic effect.

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