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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 225 - 233 (9page)
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The aim of this study was to produce porcine tetraploid (4N) parthenogenetic embryos using various methods and evaluate their developmental potential. In method 1 (M1), porcine 4N parthenogenetic embryos were obtained by inhibiting extrusion of both first (PB1) and second (PB2) polar bodies; in methods 2 (M2) and 3 (M3), 4N parthenogenetic embryos were obtained by electrofusion of 2-cell stage diploid parthenogenetic embryos derived from inhibition of PB2 or PB1 extrusion, respectively. We found no differences in the rates of cleavage or blastocyst formation or the proportion of 4N embryos among M1, M2, and M3 groups. The different methods also did not influence apoptosis rates (number of TUNEL-positive cells/number of total cells) or expression levels of apoptosis-related BAX and BCL2L1 genes. However, total cell and EdU (5-ethynyl-2’-deoxyuridine)-positive cell numbers in 4N parthenogenetic blastocysts derived from M1 were higher (p < 0.05) than those for M2 and M3 groups. Our results suggest that, although porcine 4N parthenogenetic embryos could be produced by a variety of methods, inhibition of PB1 and PB2 extrusion (M1) is superior to electrofusion of 2-cell stage diploid parthenogenetic embryos derived from inhibition of PB2 (M2) or PB1 (M3) extrusion.
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참고문헌 (21)
참고문헌 신청
Bai C / 2011 / Diploid oocyte formation and tetraploid embryo development induced by cytochalasin B in bovine / Cell Reprogram 13:37~45
Lin J / 2011 / Defects in trophoblast cell lineage account for the impaired in vivo development of cloned embryos generated by somatic nuclear transfer / Cell Stem Cell 8:371~375
Okada Y / 2007 / Complementation of placental defects and embryonic lethality by trophoblast-specific lentiviral gene transfer / Nat Biotechnol 25:233~237
Iwasaki S / 2000 / Production of live calves derived from embryonic stem-like cells aggregated with tetraploid embryos / Biol Reprod 62:470~475
Kang L / 2009 / iPS cells can support full-term development of tetraploid blastocyst-complemented embryos / Cell Stem Cell 5:135~138
Lin J / 2011 / Defects in trophoblast cell lineage account for the impaired in vivo development of cloned embryos generated by somatic nuclear transfer / Cell Stem Cell 8:371~375
Okada Y / 2007 / Complementation of placental defects and embryonic lethality by trophoblast-specific lentiviral gene transfer / Nat Biotechnol 25:233~237
Iwasaki S / 2000 / Production of live calves derived from embryonic stem-like cells aggregated with tetraploid embryos / Biol Reprod 62:470~475
Kang L / 2009 / iPS cells can support full-term development of tetraploid blastocyst-complemented embryos / Cell Stem Cell 5:135~138
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