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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 646 - 653 (8page)
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초록· 키워드
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Alcalase hydrolysis of liquid egg white was used to produce 5-hydroxytryptophan (HTP) under various conditions and investigate the sleep-potentiating activity of liquid egg white hydrolysate (LEH) on pentobarbital-induced sleep. Alcalase hydrolysis yielded the highest content of 5-HTP ($13.50{\mu}g/mL$), while neutrase hydrolysis showed the lowest 5-HTP content ($5.23{\mu}g/mL$). The liquid egg white to water ratio (1:1) was optimal for the production of 5-HTP with high amino-nitrogen (A-N) content and degree of hydrolysis. The 5-HTP, amino-nitrogen, and degree of hydrolysis increased until 24 h of hydrolysis and slightly increased thereafter during hydrolysis with 2% and 5% enzyme addition. 5-HTP administration at doses of 6 and 9 mg/kg significantly increased sleep duration and decreased sleep latency time compared to that in the control (p<0.05). LEH (150 mg/mouse), which was equivalent to 5-HTP at 6 mg/kg, significantly decreased sleep latency time and increased sleep duration time compared to that in the control (p<0.05). Oral administration of LEH showed sleep-potentiating effects because of 5-HTP. The sleep-potentiating activity of LEH may have occurred through 5-HTP in our pentobarbital-induced sleep model. LEH may be a valuable alternative to sleep enhancement and may be used as a sleep-potentiating agent.
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참고문헌 (26)
참고문헌 신청
Adler-Nissen, J / 1986 / Enzymic hydrolysis of food proteins / Elsevier applied science publishers
Benjakul, S / 1997 / Protein hydrolysates from Pacific whiting solid wastes / J. Agric. Food Chem 45:3423~3430
조대연 / 2014 / Antioxidant Effect and Functional Properties of Hydrolysates Derived from Egg-White Protein / 한국축산식품학회지 34(3):362~371
Constantinides, A / 1980 / Enzymatic modification of vegetable protein - Mechanism, kinetics, and production of soluble and partially soluble-protein in a batch reactor / Biotechnol. Bioeng 22:1543~1565
Gibbs, B. F. / 2004 / Production and characterization of bioactive peptides from soy hydrolysate and soy-fermented food / Food Res. Int 37:123~131
Benjakul, S / 1997 / Protein hydrolysates from Pacific whiting solid wastes / J. Agric. Food Chem 45:3423~3430
조대연 / 2014 / Antioxidant Effect and Functional Properties of Hydrolysates Derived from Egg-White Protein / 한국축산식품학회지 34(3):362~371
Constantinides, A / 1980 / Enzymatic modification of vegetable protein - Mechanism, kinetics, and production of soluble and partially soluble-protein in a batch reactor / Biotechnol. Bioeng 22:1543~1565
Gibbs, B. F. / 2004 / Production and characterization of bioactive peptides from soy hydrolysate and soy-fermented food / Food Res. Int 37:123~131
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